摘要
为研究苯乳酸(Phenyllactic Acid,PLA)对单增李斯特菌(Listeria monocytogenes,Lm)生物被膜的影响,采用结晶紫法检测Lm生物被膜生物量、倒置显微镜和扫描电镜观察Lm生物被膜结构;分别采用苯酚硫酸法、福林酚法和二苯胺法测定生物被膜中胞外多糖、胞外蛋白和胞外DNA的含量;利用实时荧光定量PCR检测PLA对Lm生物被膜形成相关基因转录水平的影响。结果表明:PLA对8株Lm菌株的最小抑菌浓度值均为6 mg/mL。PLA通过抑制胞外多糖和胞外蛋白的合成抑制Lm生物被膜的形成。对于Lm成熟生物被膜,PLA通过减少胞外多糖和胞外蛋白的量破坏生物被膜结构、降低生物被膜生物量。PLA还能抑制Lm的运动性。在PLA作用下Lm群体感应系统agr的转录水平显著(P<0.05)降低。作为一种天然抗菌物质,PLA在控制Lm及其生物被膜对食品污染方面具有潜在的应用价值。
To investigate the effect of phenyllactic acid(PLA)on Listeria monocytogenes biofilms,crystal violet method was used to measure the biomass of L.monocytogenes biofilms,and inverted microscope and scanning electron microscope were used to observe the structure of L.monocytogenes biofilms,the content of extracellular polysaccharide,extracellular protein and extracellular DNA in biofilm was measured by phenol-sulfuric acid method,Folin-phenol method and diphenylamine method,respectively.The effect of PLA on the transcription of genes associated with biofilm formation of L.monocytogenes was detected by real-time quantitative PCR.The results showed that the minimum inhibitory concentration values of PLA against eight L.monocytogenes strains were 6 mg/mL.PLA inhibited L.monocytogenes biofilm formation by reducing the synthesis of extracellular polysaccharides and extracellular proteins.For mature biofilms of L.monocytogenes,PLA destroyed the biofilm structure and reduced the biofilm biomass by reducing the amount of extracellular polysaccharides and extracellular proteins.PLA also inhibited the swarming motility of L.monocytogenes.The transcription levels of quorum sensing system agr in L.monocytogenes were significantly(P<0.05)decreased in the presence of PLA.As a natural antimicrobial compound,PLA had the potential application to control food contamination by L.monocytogenes and its biofilms.
作者
姜聪一
康瑞
于涛
姜晓冰
JIANG Congyi;KANG Rui;YU Tao;JIANG Xiaobing(College of Life Sciences,Henan Normal University,Xinxiang 453007,China;School of Life Sciences&Basic Medicine,Xinxiang University,Xinxiang 453000,China)
出处
《食品工业科技》
CAS
北大核心
2023年第18期164-172,共9页
Science and Technology of Food Industry
基金
河南省自然科学基金(222300420470)
河南省高等学校重点科研项目基础研究计划(222300420470)
河南师范大学国家级科研项目培育基金(20200148)。
关键词
单增李斯特菌
苯乳酸
生物被膜
胞外多聚物
Listeria monocytogenes
phenyllactic acid
biofilm
extracellular polymeric substances
