摘要
本试验通过测定桑叶提取物的体外抗氧化活性及其对黄羽肉鸡血清、肝脏抗氧化指标和肝脏抗氧化基因mRNA相对表达量的测定,旨在探究桑叶提取物的综合抗氧化能力。通过测定桑叶提取物的1,1-二苯基-2-苦基肼(DPPH)自由基、2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基和羟自由基的清除能力和还原能力评定桑叶提取物的体外抗氧化活性。选取体重相近、体况健康的42日龄雄性中速型黄羽肉鸡120羽,随机分为4组,每组6个重复,每个重复5羽鸡。对照组(CON组)饲喂基础饲粮,试验组分别在基础饲粮的基础上添加0.05%、0.10%和0.20%的桑叶提取物。试验期共42 d。结果表明:1)桑叶提取物浓度在0.2~1.5 mg/mL时对羟自由基、ABTS自由基和DPPH自由基均有清除作用,且桑叶提取物的还原能力、羟自由基清除率和ABTS自由基清除率随着桑叶提取物浓度的增加显著升高(P<0.05)。2)与CON组相比,0.05%、0.10%和0.20%组血清谷胱甘肽过氧化物酶(GPx)活性和总抗氧化能力(T-AOC)显著升高(P<0.05),血清活性氧(ROS)含量显著降低(P<0.05);0.20%组血清过氧化氢酶(CAT)活性显著升高(P<0.05)。3)与CON组相比,0.05%、0.10%和0.20%组肝脏GPx活性显著升高(P<0.05),肝脏丙二醛(MDA)和ROS含量显著降低(P<0.05);0.20%组肝脏T-AOC显著升高(P<0.05),0.10%和0.20%组肝脏CAT活性显著升高(P<0.05)。4)与CON组相比,0.10%组肝脏核因子E2相关因子2(Nrf2)mRNA相对表达量显著升高(P<0.05),0.20%组肝脏超氧化物歧化酶1(SOD1)mRNA相对表达量显著升高(P<0.05),0.10%和0.20%组肝脏CAT mRNA相对表达量显著升高(P<0.05),0.05%、0.10%和0.20%组肝脏GPx mRNA相对表达量均显著升高(P<0.05)。综上可知,桑叶提取物具有良好的体外抗氧化活性,饲粮中添加桑叶提取物可以提高黄羽肉鸡抗氧化能力,在桑叶提取物添加量以0.10%~0.20%为宜。
This experiment was conducted to study the in vitro antioxidant activity of mulberry leaf extract and its effects on serum and liver antioxidant indexes and liver antioxidant gene mRNA relative expression levels of yellow feather broilers,and to investigate the comprehensive antioxidant capacity of mulberry leaf extract.The scavenging ability and reducing ability of 1,1⁃diphenyl⁃2⁃picrylhydrazine(DPPH)radical,2,2′⁃diazobis(3⁃ethylbenzothiazoline⁃6⁃sulfonic acid)diamine salt(ABTS)radical and hydroxyl radical were analyzed to eval⁃uate the in vitro antioxidant activity of mulberry leaf extract.A total of 120 healthy 42⁃day⁃old male medium speed yellow feather broilers with similar body weight were randomly divided into 4 groups with 6 replicates per group and 5 broilers per replicate.Broilers in the control group(CON group)were fed a basal diet,and others in experimental groups were fed basal diets supplemented with 0.05%,0.10%and 0.20%mulberry leaf extract,respectively.The trial period was 42 days.The results showed as follows:1)the mulberry leaf extract had scavenging effects on hydroxyl radical,ABTS radical and DPPH radical when the concentration was 0.2 to 1.5 mg/mL,and the reducing ability,hydroxyl radical clearance rate and ABTS radical clearance rate of mul⁃berry leaf extract were significantly increased with the mulberry leaf extract concentration increased(P<0.05).2)Compared with the CON group,the serum glutathione peroxidase(GPx)activity and total antioxidant ca⁃pacity(T⁃AOC)of 0.05%,0.10%and 0.20%groups were significantly increased(P<0.05),and the serum reactive oxygen species(ROS)content was significantly decreased(P<0.05);the serum catalase(CAT)ac⁃tivity of 0.20%group was significantly increased(P<0.05).3)Compared with the CON group,the liver GPx activity of 0.05%,0.10%and 0.20%groups was significantly increased(P<0.05),and the contents of ma⁃londialdehyde(MDA)and ROS in liver were significantly decreased(P<0.05);the liver T⁃AOC of 0.20%group was significantly increase
作者
王银玲
薛玉洋
孙浩彬
南珊珊
张文举
聂存喜
WANG Yinling;XUE Yuyang;SUN Haobin;NAN Shanshan;ZHANG Wenju;NIE Cunxi(College of Animal Science and Technology,Shihezi University,Shihezi 832000,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2023年第8期5059-5068,共10页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金(31760686)。
关键词
桑叶提取物
黄羽肉鸡
体外抗氧化活性
抗氧化能力
mulberry leaf extract
yellow feather broilers
in vitro antioxidant activity
antioxidant capacity
