摘要
胞外聚合物(EPS)是一种环境友好的净水剂,高效提取EPS可以为吸附重金属离子及抗生素提供更多的吸附位点。本研究采用不同的络合剂去除二价阳离子以提高EPS的提取量,对柠檬酸钠(SC)法、草酸钠(SO)法、乙二胺四乙酸二钠(EDTA-2Na)法和酒石酸钾钠(SS)法提取的EPS特性进行对比。草酸钠法整体提取效率高,对EPS的组分和性质影响小,吸附效果好。柠檬酸钠和EDTA-2Na法对EPS的大分子成分造成不同程度的破坏,尤其是EDTA-2Na法明显改变了EPS的荧光特性。结合红外光谱和XPS分析证明EPS中各官能团与蛋白质、多糖等物质密切相关。研究为进一步发掘利用络合剂对二价阳离子络合提取胞外聚合物及后续应用提供理论依据和技术支持。
As an environmentally friendly water purification agent,an efficient extraction of extracellular polymeric substances(EPS) can provide more adsorption sites for heavy metal ions and antibiotics.In this study,different complexing agents were used to remove divalent cations to increase the extraction amount of EPS,and a comparison was made among the properties of extracted EPS by sodium citrate(SC),sodium oxalate(SO),disodium ethylenediaminetetraacetate(EDTA-2Na) and sodium potassium tartrate(SS) methods.The results showed that the sodium oxalate method had a highly overall extraction efficiency,a low impact on the composition and properties of EPS,and a good adsorption effect.The sodium citrate method and EDTA-2Na method caused different degrees of damage to the macromolecular components of EPS,especially the EDTA-2Na method obviously changed the fluorescence properties of EPS.The combination of IR spectroscopy and XPS analysis proved that the functional groups in EPS were close correlated with proteins,polysaccharides and other substances.This study can provide a theoretical basis and technical support for the future in-depth exploration of EPS extraction by complexation of divalent cations with complexing agents and the subsequent applications.
作者
费维繁
杨英
李卫华
陈玉
王娣
FEI Weifan;YANG Ying;LI Weihua;CHEN Yu;WANG Di(School of Environmental and Energy Engineering,Anhui Jianzhu University,Hefei 230000,China)
出处
《环境工程学报》
CAS
CSCD
北大核心
2023年第6期1841-1850,共10页
Chinese Journal of Environmental Engineering
基金
国家自然科学基金(81978003)
安徽省重大科技专项(201903a06020034)。
关键词
胞外聚合物
提取特性
二价阳离子
蛋白质二级结构
XPS分析
extracellular polymeric substances
extraction characteristics
divalent cations
protein secondary structure
XPS analysis
