摘要
以光合色素含量差异较大的籼稻RT和籼稻SC及其杂交衍生的RIL群体为材料,运用相关分析、主成分分析、聚类分析等方法对4个光合色素性状(叶绿素a、叶绿素b、叶绿素a/b和总叶绿素)进行综合分析和QTL定位。相关性分析结果表明,4个光合色素性状间均呈极显著正相关,叶绿素a与总叶绿素含量的相关系数最高。主成分分析结果表明,对光合色素含量影响较大的性状有叶绿素a和总叶绿素含量。聚类分析结果表明,可将239个株系分为3大类群,第Ⅰ类群、第Ⅱ类群、第Ⅲ类群的平均光合色素含量依次降低。运用复合区间作图法共检测到4个与控制光合色素性状相关的QTL,分布于水稻第4和第8染色体上,LOD值为2.01~3.14,表型贡献率的分布范围为4.52%~7.08%,加性效应均为正值,其中,在第8染色体RM6155至RM23446区间发现1个QTL簇,聚集了控制叶绿素a、叶绿素a/b和总叶绿素3个性状的QTL,该区间是影响光合色素性状的1个重要染色体区域,检测到的qChla-8、qChla/b-4、q Chla/b-8和qChlt-8可能为调控光合色素的新QTL位点。
This study utilizes RT,SC,and RIL populations derived from hybrids exhibiting substantial variations in photosynthetic pigment content.The aim was to investigate correlations and QTL mapping among four photosynthetic pigment traits(chlorophyll a,chlorophyll b,chlorophyll a/b and total chlorophyll) through correlation analysis,principal component analysis and cluster analysis.Correlation analysis revealed notable positive correlations among the four photosynthetic pigment traits,with the highest correlation coefficient observed between chlorophyll a and total chlorophyll content.Principal component analysis highlighted chlorophyll a and total chlorophyll content as pivotal factors significantly influencing photosynthetic pigment levels.Cluster analysis categorized 239 strains into three distinct groups,with their average photosynthetic pigment contents of Group Ⅰ,Group Ⅱ,Group Ⅲ decreased successively.Furthermore,employing the composite interval mapping method,four QTLs associated with photosynthetic pigment content were identified.These QTLs were located on rice chromosomes 4 and 8.The LOD values ranged from 2.01 to 3.14,and phenotypic contribution rates ranged from 4.52% to 7.08%.Importantly,all additive effects were positive.A noteworthy QTL cluster was found on chromosome 8,encompassing the RM6155 to RM23446 markers.This cluster controlled chlorophyll a,chlorophyll a/b,and total chlorophyll traits,signifying a pivotal chromosomal region influencing photosynthetic pigment characteristics.Additionally,this study discovered new QTL sites regulating photosynthetic pigments,including qChla-8,qChla/b-4,q Chla/b-8 and qChlt-8.
作者
胡超
刘玲
熊佳铭
袁红亮
于玉凤
王悦
HU Chao;LIU Ling;XIONG Jiaming;YUAN Hongiang;YU Yufeng;WANG Yue(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;College of Education,Hunan Agricultural University,Changsha,Hunan 410128,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2023年第4期383-388,共6页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家重点研发计划项目(2017YFE0194900)
湖南省自然科学基金项目(2020JJ4360)
湖南省教育厅项目(19A220)。
关键词
水稻
光合色素
主成分分析
聚类分析
QTL定位
rice
photosynthetic pigment
principal component analysis
cluster analysis
QTL mapping
