摘要
目的研究小核仁RNA宿主基因17(SNHG17)通过miR-23b-3p和锌指结构同源框蛋白1(ZHX1)调控胶质母细胞瘤(GBM)的恶性表型的作用。方法选取2020年1月至2022年10月广西壮族自治区脑科医院神经外科诊断为GBM的33例患者的手术标本,应用实时荧光定量聚合酶链式反应(qRT-PCR)技术检测GBM样本和癌旁正常组织中的SNHG17、miR-23b-3p和ZHX1的表达,通过Pearson相关分析评估SNHG17,miR-23b-3p及ZHX1之间的表达相关性。使用GBM细胞系A172和DK-MG作为细胞模型,利用细胞转染技术将细胞分为si-NC组(转染空白抑制剂)、si-SNHG17组(转染si-SNHG17#2)、si-SNHG17+miR-23b-3p inhibitor组(转染si-SNHG17#2+miR-23b-3p inhibitor),采用CCK-8和EdU法检测GBM细胞的增殖能力,使用Transwell实验评估GBM细胞的迁移和侵袭能力。使用qRT-PCR技术及Western blot实验检测SNHG17和miR-23b-3p的相互调节及两者对ZHX1的调控作用。结果GBM组织中的SNHG17和ZHX1表达水平高于癌旁正常组织,miR-23b-3p表达水平低于癌旁正常组织,差异有统计学意义(P<0.05)。在GBM组织中,SNHG17与miR-23b-3p的表达呈负相关(r=-0.711,P<0.05),而与ZHX1的表达呈正相关(r=0.648,P<0.05);miR-23b-3p和ZHX1的表达呈负相关(r=-0.637,P<0.05)。si-SNHG17组中miR-23b-3p表达高于si-NC组,GBM细胞的增殖、迁移、侵袭能力及ZHX1的表达水平均低于si-NC组,差异有统计学意义(P<0.05)。si-SNHG17+miR-23b-3p inhibitor组中miR-23b-3p表达低于si-SNHG17组,GBM细胞的增殖、迁移、侵袭能力及ZHX1的表达水平高于si-SNHG17组,差异有统计学意义(P<0.05)。结论SNHG17通过调节miR-23b-3p/ZHX1轴促进GBM细胞的增殖、迁移和侵袭进程,提示SNHG17可能是GBM的一个潜在的治疗靶点。
Objective To detect detection of small nucleolar RNA host gene 17(SNHG17)How to regulate malignant phenotype of glioblastoma(GBM)by miR-23b-3p and zinc finger structure homeobox protein 1(ZHX1).Methods Surgical specimens of 33 patients diagnosed with GBM in the Department of Neurosurgery,the Guangxi Zhuang Autonomous Region Brain Hospital from January 2020 to October 2022 were selected.The expression of SNHG17,miR-23b-3p,and ZHX1 in GBM samples and normal tissues adjacent to tumor were detected by real-time quantitative polymerase chain reaction(qRT-PCR).Pearson correlation analysis was used to evaluate the correlation between SNHG17,miR-23b-3p,and ZHX1.GBM cell line A172 and DK-MG were used as cell models,the cells were divided into si-NC group(transfected with blank inhibitor),si-SNHG17 group(transfected with si-SNHG17#2),and si-SNHG17+miR-23b-3p inhibitor group(transfected with si-SNHG17#2 and miR-23b-3p inhibitor).The proliferation capacity of GBM cells was detected by CCK-8 and EdU methods,while the migration and invasion of GBM cells were evaluated by Transwell assay.The qRT-PCR and Western blot were used to detect the mutual regulation of SNHG17 and miR-23b-3p,and their regulatory effect on ZHX1.Results The expression levels of SNHG17 and ZHX1 in GBM tissue normal tissue adjacent to cancer,the differences were statistically significant(P<0.05).In GBM tissues,SNHG17 expression was negatively correlated with the expression of miR-23b-3p(r=-0.711,P<0.05),but positively correlated with the expression of ZHX1(r=0.648,P<0.05);the expression of miR-23b-3p and ZHX1 was negatively correlated(r=-0.637,P<0.05).The expression of miR-23b-3p in the si-SNHG17 group was higher than that in the si-NC group,and the proliferation,migration,and invasion ability of GBM cells and the expression level of ZHX1 were lower than those in the si-NC group,the differences were statistically significant(P<0.05);however,the expression of miR-23b-3p in the si-SNHG17+miR-23b-3p inhibitor group was lower than that in the si-SNHG17 group,and
作者
葛北海
周伟
韦柠琳
张献
莫云
苏州
秦光平
徐国龙
GE Beihai;ZHOU Wei;WEI Ninglin;ZHANG Xian;MO Yun;SU Zhou;QIN Guangping;XU Guolong(Department of Neurology,Guangxi Zhuang Autonomous Region Brain Hospital,Guangxi Zhuang Autonomous Region,Liuzhou 545005,China;Department of Neurosurgery,Guangxi Zhuang Autonomous Region Brain Hospital,Guangxi Zhuang Autonomous Region,Liuzhou 545005,China)
出处
《中国医药导报》
CAS
2023年第23期16-22,共7页
China Medical Herald
基金
广西壮族自治区自然科学基金资助项目(2020GXNSFAA297115)。
