摘要
鞘脂不仅是细胞膜结构的重要组成成分,同样是重要的生物活性分子。本研究旨在比较分析核桃、山核桃和薄壳山核桃3种木本油料中鞘脂(Sphingolipids)的组成特性。采用UHPLC Nexera LC-30A超高效液相色谱系统进行分离,之后用Q-Exactive系列质谱仪对3种核桃中鞘脂进行质谱分析,结合LipidSearch数据库软件和脂质分子的同位素内标进行脂质鉴定。共鉴定出40种鞘脂分子,包括25种神经酰胺(ceramide,Cer)、8种鞘糖脂(glucosyl-ceramide,CerG1)、4种鞘氨醇(sphingosine,So)和3种鞘磷脂(sphingomyelin,SM)。三种核桃中的鞘脂均含有14种饱和脂肪酸(saturated fatty acids,SFA),5种单不饱和脂肪酸(monounsaturated fatty acids,MUFA),7种多不饱和脂肪酸(polyunsaturated fatty acids,PUFA)。其中,17种脂质分子可作为区分三种核桃的显著差异脂质分子。此外,聚类分析结果显示薄壳山核桃和山核桃的鞘脂组成更为相似,此结果与核桃的系统分类一致。本实验结果可为核桃类坚果在针对鞘脂的研究、产品开发和利用方面提供数据依据。
Sphingolipids are important components of cell membranes and bioactive molecules.The purpose of this study was to compare and analyze the composition of sphingolipids in walnut,hickory and pecan.Ultra high per-formance liquid chromatography was used to separate sphingolipids,and mass spectrometry was performed by QExactive Plus mass spectrometer.Lipidsearch database software combined with internal lipid standard mixture were used to identify lipid molecules.The results showed that a total of 40 sphingolipid molecules were identified,includ-ing 25 ceramides(Cer),8 glucosylceramides(CerG1),4 sphingosines(So),and 3 sphingomyelins(SM).17 sphingolipid molecules could distinguish the three kinds of nuts as significantly different lipid molecules.The result of cluster analysis is consistent with that of system classification.There contained 14 saturated fatty acids(SFA),5 monounsaturated fatty acids(MUFA),and 7 polyunsaturated fatty acids(PUFA).It could provide data basis for the research,product development and utilization of sphingolipids of three nuts.
作者
谭文玥
许梦洋
佟海英
贾晓东
TAN Wen-yue;XU Meng-yang;TONG Hai-ying;JIA Xiao-dong(Jiangsu Key Laboratory for the Research and Utilization of Plant Resources,Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China;Nanjing University of Chinese Medicine,Nanjing 210023,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2023年第4期863-872,共10页
Chinese Journal of Oil Crop Sciences
基金
江苏省林业科技创新与推广项目(LYKJ-句容[2020]01)
江苏省科技厅重点研发计划项目(BE2021349)。
关键词
超高效液相色谱
核桃
山核桃
薄壳山核桃
鞘脂
脂肪酸
ultra high performance liquid chromatography
walnut
hickory
pecan
sphingolipid
fatty acid
