摘要
【目的】探索黄金凤可培养内生细菌资源并筛选具有促生活性的菌株。【方法】以野生黄金凤为研究材料,采用3种分离处理和5种不同培养基对其内生细菌进行分离,并通过16SrRNA基因序列对分离得到的内生细菌进行鉴定;测定其ACC脱氨酶活性、产IAA能力、溶磷及产铁载体能力。【结果】从黄金凤中分离去重后获得39株内生细菌,分属于5个目、7个科和8个属,其中以100倍稀释悬浮法(X法)分离效果最佳,共获得6个菌属;最好的分离培养基为M-7培养基(5号培养基),共分离获得5个菌属。促生能力测定结果表明:39株内生细菌均具有产ACC脱氨酶能力,其中菌株SWFU195产ACC脱氨酶能力最强,为11.0390U/mg;27株内生细菌具产IAA的能力;24株具有溶磷能力;28株内生细菌具产铁载体能力;39株黄金凤内生细菌中有14株内生细菌同时具有溶磷、产ACC脱氨酶、产IAA和产铁载体能力。【结论】野生黄金凤植株中蕴藏着丰富的内生细菌资源及促生活性菌株,为野生黄金凤资源的保护利用及其促生活性菌株的开发应用提供了一定的基础数据和理论依据。
【Objective】This paper aims to explore the resources of cultivable endophytic bacteria and screen the life-promoting strains in Impatiens siculifer.【Method】The endophytic bacteria of wild Impa⁃tiens siculifer were isolated by three isolation methods and five different culture media,and the isolated endophytic bacteria were identified by 16S rRNA-gene sequences.The activities of ACC deaminase,synthesis of IAA,the abilities of dissolving phosphorus and producing iron carrier were investigated.【Result】A total of 39 endophytic bacteria were obtained,belonging to 5 orders,7 families and 8 genera.The suspension method(X method)had the best separation effect,and a total of 6 genera were obtained.The best isolation medium was M-7 glycerol-asparagine medium(No.5 medium),and 5 genera were isolated.Based on the testing results of growth-promoting abilities,it showed that all 39 endophytic bacteria had the ability to produce ACC deaminase,and the strain SWFU195 had the highest ability to produce ACC deaminase among them,which was 11.0390 U/mg;27 strains were capable of producing IAA;24 strains had phos phorus solubilization ability;28 strains had siderophore production capacity;Among 39 strains of endophytic bacteria,14 strains of endophytic bacteria also had phosphorus soluble,produce ACC deaminase,synthesis of IAA and produce iron carrier simultaneously.【Conclusion】Rich endogenous bacterial resources and life-promoting strains were found in I.siculifer,which provided certain basic data and theoretical basis for its development and utilization and its growth-promoting endophytic bacteria strains.
作者
王梦雨
贾颜
李茜
李新艺
李文祥
黄海泉
黄美娟
WANG Mengyu;JIA Yan;LI Qian;LI Xinyi;LI Wenxiang;HUANG Haiquan;HUANG Meijuan(College of Landscape Architecture and Horticulture Sciences,Southwest Forestry University/Southwest Research Center for Engineering Technology of Landscape Architecture(State Forestry and Grassland Administration)/Yunnan Engineering Research Center for Functional Flower Resources and Industrialization/Research and Development Center of Landscape Plants and Horticulture Flowers,Southwest Forestry University,Kunming 650224,China)
出处
《四川农业大学学报》
CSCD
北大核心
2023年第4期650-657,共8页
Journal of Sichuan Agricultural University
基金
云南省重大科技专项(202102AE090052)
国家自然科学基金项目(32060364、32060366、31860230、31560228)
云南省高校园林植物与观赏园艺科技创新团队(51700204)
云南省园林植物遗传改良与高效繁育博士生导师团队和云南省中青年学术和技术带头人培养项目(2018HB024)。
关键词
黄金凤
内生细菌
分离
促生活性
Impatiens siculifer
endophytic bacteria
isolation
growth-promoting activities
