摘要
【目的】探究青花椒抗逆调控基因ZaMBF1基因家族特征和非生物胁迫下的表达模式,为青花椒抗性育种提供重要的基因来源和理论基础。【方法】结合近年发表的青花椒基因组数据,综合利用生物信息学研究方法和荧光定量PCR技术,解析了多蛋白侨联因子MBF1家族(multiprotein bridging factor 1)的核酸和蛋白序列特征,探究了ZaMBF1s对盐胁迫和脱落酸处理后的响应模式。【结果】青花椒共有3个MBF1家族成员,分别为ZaMBF1a、ZaMBF1b和ZaMBF1c。其中ZaMBF1a和ZaMBF1b编码序列长度均为429 bp,分别位于26号和32号染色体,而ZaMBF1c编码序列长度为453 bp,位于4号染色体;青花椒ZaMBF1s均为亲水性的不稳定蛋白,其核酸序列和对应的启动子序列均含有丰富的WRKY、bHLH等抗逆调控基因结合位点和顺式作用元件;ZaMBF1家族基因在NaCl外源处理3h后即可快速响应,并在处理后12h再次极显著上调,尤其是ZaMBF1c的上调最为显著;同时,外源处理12h也是ZaMBF1家族基因响应ABA处理的重要时间点。【结论】青花椒ZaMBF1家族成员可以参与抗逆调控过程,并对外源盐和脱落酸处理具有显著响应。本研究所获结果对于探究ZaMBF1基因的分子功能,揭示青花椒抗逆性的调控机制提供了丰富的数据支撑。
【Objective】To explore the characters of ZaMBF1 gene family and its expression patterns in different abiotic stresses,and provide an important gene source and theoretical basis for resistance breeding in Zanthoxylum armatum.【Method】According to the public genome data of Z.armatum,this study comprehensively conduct the bioinformatics analysis of ZaMBF1 family(multiprotein bridging factor 1),including nucleic acid and protein sequence characters,and the expression patterns of ZaMBF1s were also explored in response to salt stress and ABA treatment using quantitative reverse transcription PCR technology.【Result】A total of three MBF1 members were detected in Z.armatum,named ZaMBF1a,ZaMBF1b and ZaMBF1c.Both the coding sequences of ZaMBF1a and ZaMBF1b were 429 bp and located on chromosomes No.26 and No.32,respectively.However,the coding sequence of ZaMBF1c was 453 bp,located on chromosome No.4.Besides,all of the ZaMBF1 were belonged to hydrophilic and unstable proteins,and it was enriched a plenty of WRKY,bHLH and other stress-resistant regulatory binding sites and cis-acting elements in the ZaMBF1s nucleic acid sequences and the corresponding promoter sequences.Meanwhile,the ZaMBF1 genes rapidly responded at 3 h after exogenous NaCl treatment,and were significantly up-regulated at 12 h once again,especially the ZaMBF1c.Additionally,the 12 h after exogenous treatment was also an crucial time point for ZaMBF1 family genes in response to ABA treatment.【Conclusion】The ZaMBF1 family members could be involved in stress regulation,and respond significantly to exogenous salt and ABA treatment.The present results will provide enough support for exploring the molecular functions of ZaMBF1 genes and revealing the regulatory mechanism of stress resistance in Zanthoxylum armatum.
作者
王寒葶
张雨
李豪
郑皓
田源成
朱舜棱
许博洋
王景燕
龚伟
惠文凯
WANG Hanting;ZHANG Yu;LI Hao;ZHENG Hao;TIAN Yuancheng;ZHU Shunling;XU Boyang;WANG Jingyan;GONG Wei;HUI Wenkai(College of Forestry,Sichuan Agricultural University,Sichuan Province Key Laboratory of Ecological Forestry Engineering on the Upper Reaches of the Yangtze River,Chengdu 611130,China)
出处
《四川农业大学学报》
CSCD
北大核心
2023年第4期592-601,共10页
Journal of Sichuan Agricultural University
基金
国家重点研发计划项目(2020YFD1000700、2018YFD1000605)
四川省科技基金计划项目(2020YJ0130)
四川省“十四五”林木育种攻关项目(2021YFYZ0032)。
