摘要
目的 测定秦巴山不同产区连翘叶内多酚类化合物的含量,并进行连翘叶质量评价。方法 采用HPLC法测定连翘苷、连翘酯苷A和芦丁的含量,采用紫外-可见分光光度法测定总黄酮的含量。以10批秦巴山不同产区连翘叶药材中上述4种成分的含量为指标,采用TOPSIS法综合评价连翘叶药材的整体质量。结果 TOPSIS法分析结果显示,10批不同产地连翘药材的相对贴近度分别为0.421 6、0.328 1、0.432 3、0.275 9、0.268 8、0.824 1、0.384 1、0.779 3、0.464 8和0.779 4。质量排名前3位的连翘药材分别为房县化龙镇、竹山麻家渡桂花村和张湾区黄龙镇青石村。结论 秦巴山所有产区连翘叶中3种多酚类化合物均达到2015版《中国药典》规定,为其合理的开发和利用提供理论依据。
Objective To determine the content of polyphenols in the Forsythia suspense leaves from different producing areas in Qinba Mountain Areas,evaluate the quality of Forsythia suspense leaves.Methods The HPLC was used to measure the content of forsythin,forsythiaside A and rutin,UV-Vis spectrophotometry was used to determine the content of total flavonoids.Based on the contents of above four components in 10 batches of Forsythia suspense leaves from different producing areas in Qinba Mountain Areas,TOPSIS method was used to evaluate the overall quality of Forsythia suspense leaves.Results The TOPSIS analysis results showed that the relative closeness of 10 batches of Forsythia suspense leaves from different producing areas were 0.4216,0.3281,0.4323,0.2759,0.2688,0.8241,0.3841,0.7793,0.4648 and 0.7794,respectively.The top three samples in the quality evaluation were collected from Hualong Town of Fangxian County,Guihua Village of Majiadu in Zhushan County and Qingshi Villag of Huanglong Town in Zhangwan District.Conclusion The contents of three kinds of polyphenols compounds in Forsythia suspense leaves from all producing areas in Qinba Mountain Areas meet the requirements of the 2015 edition of Chinese Pharmacopoeia,which provides a theoretical basis for its rational development and utilization.
作者
骆霞
赵利军
祝宇
魏娟
周灿
张晓燕
LUO Xia;ZHAO Lijun;ZHU Yu;WEI Juan;ZHOU Can;ZHANG Xiaoyan(Department of Pharmacy,Shiyan People's Hospital,People's Hospital Affiliated to Hubei University of Medicine,Shiyan Hubei,442000,China;Hubei Key Laboratory of Wudang Local Chinese Medicine Research,Shiyan Hubei,442000,China)
出处
《职业与健康》
CAS
2023年第15期2017-2020,2025,共5页
Occupation and Health
基金
湖北省教育厅科研计划项目(B2020110)
十堰市市级引导性科研项目(22Y68)。
