摘要
目的探究妊娠期甲状腺功能减退(简称“妊娠期甲减”)患者血清微小RNA-124(microRNA-124,miR-124)、miR-146a及miR-326表达水平及与甲状腺功能及不良妊娠结局的相关性。方法回顾性分析2017年6月至2019年6月西安医学院第二附属医院内分泌科进行妊娠期甲减治疗的患者为观察组(n=121),对照组为同期来本院建卡正常妊娠的孕妇(n=120)。采用实时荧光定量PCR(quantitative real-time PCR,RT-qPCR)检测miR-124、miR-146a及miR-326血清表达水平;比较两组受试者血清促甲状腺激素(thyroid stimulating hormone,TSH)、血清游离三碘甲状腺原氨酸(free triodothyronine,FT3)、游离T4(FT4)、甲状腺过氧化物酶抗体(thyroid peroxidase antibody,TPOAb)、促甲状腺激素受体抗体(thyrotropin receptor antibodies,TRAb)、甲状腺微粒体抗体(thyroidmicrosomal antibody,TMAb)及甲状腺球蛋白抗体(thyroglobulin antibodies,TGAb)水平差异。采用Spearman和Pearson相关性分析探究miR-124、miR-146a及miR-326表达及与甲状腺激素各指标的相关性;采用×检验miR-124、miR-146a及miR-326水平与治疗后不良妊娠结局的相关性。结果与对照组健康孕妇相比,观察组妊娠期甲减孕妇miR-146a(0.98±0.12vs2.71±0.34)及miR-326(1.09±0.22vs2.11±0.32)血清表达上调,miR-124表达下调(1.46±0.23vs0.56±0.10),差异均有统计学意义(P<0.05);观察组孕妇TSH、TGAb、TRAb、TMAb、TPOAb高于对照组孕妇,FT3、FT4低于对照组孕妇(P<0.05);治疗后FT4、TGAb、TPOAb水平在miR-124、miR-146a及miR-326高表达组及低表达组之间有显著差异(P<0.05);miR-124表达水平与FT3、FT4呈显著正相关,与TSH、TGAb、TRAb、TMAb、TPOAb呈显著负相关,miR-146a、miR-326表达与FT3、FT4呈显著负相关,与TSH、TGAb、TRAb、TMAb、TPOAb呈显著正相关(P<0.05)。结论miR-124、miR-146a及miR-326可作为妊娠期甲减疗效评估有关,并可作为不良妊娠结局的预测因子。
Objective To exploring the correlation between the expression levels of microRNA-124(miR-124),miR-146a,and miR-326 in serum of patients with gestational hypothyroidism and thyroid function and ad-verse pregnancy outcomes.Methods Patients who came to our hospital for gestational hypothyroidism treatment from Jun.2017 to Jun.2019 were selected as the observation group(n=121),while the control group was pregnant women who came to our hospital for normal pregnancy during the same period(n=120).Quantitative real time PCR(RT-qPCR)was used to detect the serum expression levels of miR-124,miR-146a,and miR-326.The serum thy-roid hormone(TSH),free triodothyronine(FT3),free T4(FT4),thyroid peroxidase antibody(TPOAb),thyroid hormone receptor antibodies(TRAb)were compared.The levels of thyroid microsomal antibody(TMAb)and anti-thyroid autoantibodies(TGAb)were dfferent.Spearman and Pearson correlation analysis were used to explore the correlation between the expression of miR-124,miR-146a and miR-326 and the indicators of thyroid hormone.Chi-square was used to examine the correlation between the levels of miR-124,miR-146a and miR-326 and adverse pregnancy outcomes fter treatment.Results Compared with the control group of healthy pregnant women,the ob-servation group showed an upregulation of miR-146a(0.98±0.12 vs 2.71±0.34)and miR-326(1.09±0.22 vs 2.11±0.32)serum expression,while the expression of miR-124 was down regulated(1.46±0.23 vs 0.56±0.10),with statistically significant differences(P<0.05).The TSH,TGAb,TRAb,TMAb,and TPOAb in the observation group were higher than those in the control group,while FT3 and FT4 were lower than those in the control group(P<0.05).After treatment,FT4,TGAb and TPOAb levels were significantly different between miR-124,miR-146a and miR-326 high expression groups and low expression groups(P<0.05).The expression level of miR-124 was significantly positively correlated with FT3 and FT4,and significantly negatively correlated with TSH,TGAb,TRAb,TMAb and TPOAb,while the expression level of miR-
作者
林源
郭亚菊
Lin Yuan;Guo Yaju(Department of Endocrinology,the Second Affiliated Hospital of Xi'an Medical College,Xi'an 710038,China)
出处
《中华内分泌外科杂志》
CAS
2023年第4期450-454,共5页
Chinese Journal of Endocrine Surgery
基金
西安医学院第二附属医院院级课题(19KY0106)。
