摘要
目的:研究SRY盒转录因子7( SOX7)基因甲基化在肾癌中的临床价值及对肾癌细胞增殖、迁移和侵袭的影响。 方法:选择80例肾癌患者(肾癌组)及50例肾脏良性疾病患者(对照组)为研究对象。设计合成寡核苷酸序列(MON、UMON和CON)并转染至肾癌细胞A498。采用甲基特异性PCR检测肾癌患者(肿瘤组织、癌旁组织)和对照组肾脏组织中 SOX7基因甲基化状态,分析 SOX7基因甲基化与临床病理因素的关系,MTT法检测 SOX7基因甲基化对肾癌细胞增殖的影响,Transwell小室法检测 SOX7基因甲基化对肾癌细胞迁移和侵袭的影响。 结果:肾癌组患者肿瘤组织 SOX7基因甲基化率高于癌旁组织和对照组肾脏组织,差异有统计学意义(χ^(2)=67.522, P<0.05)。肾癌细胞Caki-1、786-O、769-P中 SOX7基因呈甲基化状态,而在A498细胞中呈未甲基化状态。肾癌组患者肿瘤组织 SOX7基因甲基化率在性别、年龄及病理类型方面比较差异无统计学意义(均 P>0.05),在分化程度、肿瘤最大径、淋巴结转移、肿瘤数量及肿瘤分期方面比较差异均有统计学意义(均 P<0.05)。转染MON后可成功诱导A498细胞 SOX7基因甲基化,MON组A498细胞第1~7天细胞活力高于UMON组和CON组( P<0.05)。 结论:SOX7基因甲基化可促进肾癌细胞增殖、迁移和侵袭,在肾癌的病情及预后评估中具有重要临床价值。
ObjectiveTo study the clinical value of SRY-Box transcription factor 7(SOX7)gene methylation in renal cancer and its effect on the biological behavior of renal cancer cells.Methods80 patients with renal cancer(the kidney cancer group)and 50 patients with benign renal disease(the control group)were selected as the research subjects.Synthetic oligonucleotide sequences(MON,UMON,and CON)were designed and transfected into A498 renal carcinoma cells.Methyl-specific PCR was used to detect the methylation status of the SOX7 gene in the tumor and adjacent tissue of the kidney cancer group as well as in the renal tissue of the control group.The relationship between SOX7 gene methylation and clinicopathological factors was analyzed.The migration and invasion of A498 renal cancer cells in the MON group,UMON group,and CON group were detected by the Transwell chamber.ResultsThe SOX7 gene methylation rate in tumor tissue of the kidney cancer group was significantly higher than that of the control group and the adjacent tissue,and the difference was statistically significant(χ^(2)=67.522,P<0.05).The SOX7 gene is methylated in renal cancer cell lines such as Caki-1,786-O,769-P,while it is unmethylated in A498 renal cancer cells.There were no statistical differences in the SOX7 gene methylation rate in the tumor tissue of the renal cancer group in terms of gender,age,or pathological type(all P>0.05).There were statistical differences in the degree of differentiation,maximum tumor diameter,lymph node metastasis,tumor number,and TNM staging in the renal cancer group in terms of tumor tissue SOX7 gene methylation rate(all P<0.05).After transfection with MON,the SOX7 gene methylation of A498 renal cancer cells could be successfully induced,and the day-1 to day-7 cell viability,cell migration,and invasion numbers of A498 renal cancer cells in the MON group were significantly higher than those in the UMON group and the CON group(P<0.05).ConclusionsHypermethylation of the SOX7 gene can promote the proliferation,migration,and invasion of
作者
王娟
刘俊萍
Wang Juan;Liu Junping(Department of Nephrology,Xianyang Central Hospital,Xianyang 712000,China;Dialysis Center,Xianyang Central Hospital,Xianyang 712000,China)
出处
《国际生物医学工程杂志》
CAS
2023年第3期226-230,共5页
International Journal of Biomedical Engineering
