摘要
以低共熔溶剂为提取溶剂,用球磨法从毛竹叶中提取黄酮碳苷类化合物,采用高效液相色谱-飞行时间质谱(LC-Q-TOF-MS)测定竹叶中4种黄酮碳苷(异荭草苷、荭草苷、异牡荆苷和牡荆苷)的含量,通过单因素试验和正交试验,对低共熔溶剂的含水量、提取时间、液料比等因素进行考察。结果表明,从竹叶中提取黄酮碳苷的最佳工艺为:低共熔溶剂(氯化胆碱和乙二醇构成)含水量50%、提取时间150 s、液料比6∶1(mL∶g)。在该条件下,从竹叶中提取4种黄酮碳苷的总提取得率为1.55 mg/g,其中,异荭草苷0.99 mg/g、荭草苷0.23 mg/g、牡荆苷0.04 mg/g、异牡荆苷0.29 mg/g。该方法提取得率比浸渍提取法提高了7.6%,且节约了时间和有机溶剂。利用该优化工艺,结合LC-Q-TOF-MS检测,成功应用于以毛竹叶为原料制成竹茶样品的黄酮碳苷提取与测定。
Ball mill method was used to extract flavone C-glycosides from bamboo leaves using deep eutectic solvents(DESs) as extraction solvent.The contents of four flavone C-glycosides(isoorientin,orientin,isovitexin and vitexin) in bamboo leaves were determined by liquid chromatography quadrupole time-of-flight mass spectrometry(LC-Q-TOF-MS).By single factor experiments and orthogonal design,the parameters including water content,extraction time and liquid-solid ratio of DESs were investigated.The results showed that the optimized extraction process of flavone C-glycosides from bamboo leaves was as follows:DESs(composed of choline chloride and ethylene glycol) water content of 50%,extraction time of 150 s,and liquid-solid ratio of 6 ∶1(mL ∶g).Under these conditions,the total extraction yield of four flavone C-glycosides from bamboo leaves was 1.55 mg/g,including isoorientin of 0.99 mg/g,orientin of 0.23 mg/g,vitexin of 0.04 mg/g and isovitexin of 0.29 mg/g.The extraction yield of this method was 7.6% higher than that of immersion extraction,and the time and organic solvent were saved.This optimized technology combined with LC-Q-TOF-MS detection was successfully applied to the extraction and determination of flavone C-glycosides in bamboo tea samples made from bamboo leaves.
作者
刘亚倩
王进
汤锋
LIU Yaqian;WANG Jin;TANG Feng(International Centre for Bamboo and Rattan,Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo&Rattan Science and Technology,Beijing 100102,China)
出处
《林产化学与工业》
CAS
CSCD
北大核心
2023年第4期60-66,共7页
Chemistry and Industry of Forest Products
基金
“十三五”国家重点研发计划(2016YFD0600801)。
关键词
黄酮碳苷
球磨法
低共熔溶剂
提取
竹叶
flavone C-glycosides
ball mill
deep eutectic solvents
extraction
bamboo leaves
