摘要
目的:探讨长链非编码RNA(lnc RNA)GATA3反义RNA1(GATA3-AS1)靶向mi R-515-5p对儿童急性淋巴细胞白血病(ALL)细胞增殖和凋亡的影响。方法:RT-q PCR检测对照者和ALL患儿血浆中GATA3-AS1和mi R-515-5p的表达水平。将人ALL细胞Jurkat分为si-GATA3-AS1、si-NC、mi R-NC、mi R-515-5p、si-GATA3-AS1+anti-mi R-NC和si-GATA3-AS1+anti-mi R-515-5p共6组。CCK-8法检测细胞增殖,流式细胞术分析细胞凋亡,双荧光素酶报告实验用于确定GATA3-AS1和mi R-515-5p的靶向关系。结果:ALL患儿血浆中GATA3-AS1表达水平显著高于对照者(P<0.001),mi R-515-5p表达水平显著低于对照者(P<0.001)。与si-NC组比较,si-GATA3-AS1组细胞抑制率、凋亡率、mi R-515-5p表达水平显著升高(P<0.001)。与mi R-NC组比较,mi R-515-5p组细胞抑制率、凋亡率显著升高(P<0.001)。GATA3-AS1与mi R-515-5p直接特异性结合。与si-GATA3-AS1+anti-mi R-NC组比较,si-GATA3-AS1+anti-mi R-515-5p组细胞抑制率、凋亡率显著降低(P<0.001)。结论:下调GATA3-AS1可通过靶向上调mi R-515-5p的表达抑制儿童ALL细胞增殖,并诱导细胞凋亡。
Objective:To investigate the effects of long non-coding RNA(lncRNA)GATA3 antisense RNA 1(GATA3-AS1)targeting miR-515-5p on the proliferation and apoptosis of childhood acute lymphoblastic leukemia(ALL)cells.Methods:RT-qPCR was used to determine the expression of GATA3-AS1 and miR-515-5p in the plasma of controls and ALL children.Human ALL cells Jurkat were divided into si-GATA3-AS1,si-NC,miR-NC,miR-515-5p,si-GATA3-AS1+anti-miR-NC and si-GATA3-AS1+anti-miR-515-5p groups.CCK-8 assay was used to detect the cell proliferation,and flow cytometry was used to detect the cell apoptosis.The targeting relationship between GATA3-AS1 and miR-515-5p was determined by dual-luciferase reporter assay.Results:The expression level of GATA3-AS1 in the plasma of ALL children was significantly higher than that of controls(P<0.001),while the expression level of miR-515-5p was significantly lower than that of controls(P<0.001).Compared with the si-NC group,the cell inhibition rate,apoptosis rate,and miR-515-5p expression level in si-GATA3-AS1 group were significantly increased(P<0.001).Compared with the miR-NC group,the cell inhibition rate and apoptosis rate in miR-515-5p group were significantly increased(P<0.001).GATA3-AS1 could directly and specifically bind to miR-515-5p.Compared with the si-GATA3-AS1+anti-miR-NC group,the cell inhibition rate and apoptosis rate in si-GATA3-AS1+anti-miR-515-5p group were significantly decreased(P<0.001).Conclusion:Down-regulation of GATA3-AS1 can inhibit proliferation and induce apoptosis of childhood ALL cells by targeting up-regulation of miR-515-5p expression.
作者
秦彦
韵雪雪
郑忠梅
徐倩
左立旻
QIN Yan;YUN Xue-Xue;ZHENG Zhong-Mei;XU Qian;ZUO Li-Min(Department of Emergency,The Affiliated Women's and Children's Hospital,School of Medicine,University of Electronic Science and Technology of China·Chengdu Women's and Children's Central Hospital,Chengdu 611731,Sichuan Province,China;Department of Clinical Laboratory,The Affiliated Women's and Children's Hospital,School of Medicine,University of Electronic Science and Technology of China·Chengdu Women's and Children's Central Hospital,Chengdu 611731,Sichuan Province,China;Department of Pediatric Hematology and Oncology,The Affiliated Women's and Children's Hospital,School of Medicine,University of Electronic Science and Technology of China·Chengdu Women's and Children's Central Hospital,Chengdu 611731,Sichuan Province,China)
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2023年第4期1032-1037,共6页
Journal of Experimental Hematology
基金
四川省卫生和计划生育委员会科研课题(17PJ271)。
