摘要
本试验以蕨菜干为原料,采用超声辅助法提取蕨菜粗多糖,计算提取率。再用Sevage法、大孔树脂、透析法进行纯化,得到纯化蕨菜多糖。测定了蕨菜多糖的化学成分和理化性质。采用红外光谱、刚果红试验、高效液相色谱、高碘酸氧化、碘-碘化钾反应等方法对蕨菜多糖的结构进行了表征,通过测定蕨菜多糖对DPPH自由基、ABTS+自由基的清除能力和FRAP能力评价了蕨菜多糖的体外抗氧化能力。试验结果表明,蕨菜粗多糖的提取率为8.91%,纯化后蕨菜多糖含量为76.95%,总酚含量为1.03%,黄酮含量为1.90%,硫酸根含量为21.38%,糖醛酸含量为29.26%。红外光谱结果表明,蕨菜多糖具有多糖类物质的典型特征吸收峰,以及含有糖醛酸与部分吡喃糖。单糖组成结果表明,蕨菜多糖是一个杂多糖,由10种单糖组成。蕨菜多糖侧链较长、支链较多,链状结构复杂,无三股螺旋结构。体外抗氧化试验结果表明,蕨菜多糖具有极强的ABTS+自由基清除能力,可达92.61%,具有良好的铁离子还原能力,以Trolox当量表示为40.15±1.40 mg/g,以及很强的DPPH自由基清除能力。上述结果将为进一步研究蕨菜多糖的构效关系提供依据,并为蕨菜资源的合理利用提供理论参考。
In this experiment,dried bracken was used as the raw material,and an ultrasound-assisted method was used to extract and calculate the extraction rate of bracken crude polysaccharides.The bracken polysaccharides were then purified using the Sevage method,macroporous resin,and dialysis method.Chemical composition and physicochemical properties of bracken polysaccharides were determined.Infrared spectroscopy,Congo red test,high performance liquid chromatography,periodic acid oxidation,iodine-potassium iodide reaction,and other methods were used to characterize the structure of bracken polysaccharides,and the antioxidant capacity of bracken polysaccharides on DPPH free radicals,ABTS+free radical scavenging ability,and FRAP ability were measured in vitro.The extraction rate of bracken crude polysaccharides was 8.91%,the content of purified bracken polysaccharides was 76.95%,the total phenolic content was 1.03%,the content of flavonoids was 1.90%,the sulfate content was 21.38%,and the content of uronic acid was 29.26%,according to the experimental results.The infrared spectroscopy results revealed that bracken polysaccharides had typical polysaccharide absorption peaks,as well as uronic acid and partial pyranose.The monosaccharide composition results revealed that bracken polysaccharide was a heteropolysaccharide made up of ten monosaccharides.Long side chains,more branched chains,complex chain structure,and no three-stranded helix structure of bracken polysaccharide.In vitro antioxidant testing revealed that bracken polysaccharides had a high ABTS+radical scavenging ability(up to 92.61%),a good iron ion reduction ability(40.15±1.40 mg/g in Trolox equivalent),and a high DPPH free radical scavenging ability.The above findings will serve as a foundation for further research into the structure-activity relationship of bracken polysaccharides,as well as a theoretical reference for the rational use of bracken resources.
作者
刘恒
张秀玲
李坤
薄艳秋
李佳旭
LIU Heng;ZHANG Xiuling;LI Kun;BO Yanqiu;LI Jiaxu(College of Food Science,Northeast Agricultural University,Harbin 150030,China)
出处
《食品工业科技》
CAS
北大核心
2023年第17期51-58,共8页
Science and Technology of Food Industry
基金
黑龙江省蔬菜现代产业技术协同创新推广体系岗位专家资金(HNWJZTX201701)
东北农业大学“双一流”学科团队“特色果蔬的贮藏加工与示范”(107-54941312)。
关键词
蕨菜
多糖
纯化
结构表征
抗氧化
bracken
polysaccharide
purification
structural characterization
antioxidant
