摘要
灰茶尺蠖核型多角体病毒(Ectropis grisescens Nucleopolyhedrovirus,EgNPV)是灰茶尺蠖幼虫的重要病原微生物。为明确其对茶树重要害虫尺蠖类的两个近缘种灰茶尺蠖Ectropis grisescens Warren和茶尺蠖E.obliqua Prout的致病性,进行了一系列的生物测定。结果表明,EgNPV对2龄灰茶尺蠖和茶尺蠖幼虫的剂量对数-死亡机率值回归方程和LC50分别为y=0.943x+0.3582,LC50=8.3×10^(4) PIB/mL;y=0.663x+1.614,LC50=1.3×10^(5) PIB/mL。当使用不同浓度EgNPV处理不同龄期灰茶尺蠖和茶尺蠖幼虫时发现,两种幼虫致死率趋势大致相同;在虫龄一致时,达到相同的致病率,灰茶尺蠖所需的时间比茶尺蠖平均短一天。使用2×106 PIB/mL浓度处理不同代别灰茶尺蠖和茶尺蠖2龄幼虫,在第1、2和第6代,EgNPV对两种幼虫的致死率均大于80%。上述结果表明灰茶尺蠖核型多角体病毒对灰茶尺蠖、茶尺蠖的幼虫都比较敏感,具有致死能力。这为灰茶尺蠖核型多角体病毒的合理应用提供理论基础。
Ectropis grisescens Nucleopolyhedrovirus(EgNPV)is an important pathogenic microorganism of Ectropis grisescens Warren.A series of bioassays of E.grisescens and E.obliqua from two related species of tea were analyzed to determine its pathogenicity.Results showed that the LD-P equation for the 2nd instar larvae of E.grisescens and E.obliqua was y=0.943x+0.3582;y=0.663x+1.614.And the value of LC50 for the 2nd instar larvae was 8.3×10^(4) PIB/mL and 1.3×10^(5) PIB/mL.The mortality trend for 1st―3rd instar larvae of E.grisescens and E.obliqua infected by different concentrations of EgNPV were roughly the same.Reaching the same pathogenicity,the E.grisescens was one day faster than the E.obliqua when at the same age.The death rate of EgNPV was more than 80%to both 2nd instar larvae of 1st,2nd and 6th generations when infected with EgNPV in the concentration of 2×10^(6) PIB/mL.The results provided a basis for effectively improving the control effect of EgNPV.
作者
谭荣荣
陈勋
黄丹娟
王红娟
毛迎新
TAN Rongrong;CHEN Xun;HUANG Danjuan;WANG Hongjuan;MAO Yingxin(Institute of Fruit and Tea of Hubei Academy of Agricultural Sciences/Fruit and Tea Division of Hubei Innovation Center of Agricultural Science of Techonology,Wuhan 430064,China)
出处
《中国生物防治学报》
CSCD
北大核心
2023年第3期684-689,共6页
Chinese Journal of Biological Control
基金
大别山区茶叶提质增效技术集成与应用示范(2021YFD1601103)。
