摘要
【目的】研究尾叶桉(Eucalyptus urophylla)不同无性系在相同培养基上不同时期基因的差异表达情况,为解析尾叶桉不同无性系间生根差异、挖掘不同尾叶桉无性系生根响应相关基因功能提供依据。【方法】以在同一生根培养基上生根启动差异较大、继代次数相同的尾叶桉无性系各1个,根据生根启动快的无性系基部形态差异,分别取0、1、4和6 d尾叶桉无性系基部2 mm材料进行转录组测序分析,运用基因差异表达分析、Cluster聚类分析、基因本体分析(GO分析)和KEGG分析,获得导致尾叶桉不同无性系生根差异的差异表达基因。【结果】共获得20287个差异表达基因(DEGs),显著聚类在4个子聚类中。其中,表达模式呈上升趋势的DEGs在生根启动快的无性系中,其数量远远高于在生根启动慢的无性系,而在1与0 d时表达量变化不显著的基因在生根启动慢的无性系中其数量远高于在生根启动快的无性系中。GO分析显示,转移至IBA培养基上后,子聚类17的DEGs在生根启动快的无性系中显著富集到细胞周期相关路径中,而在生根启动慢的无性系中显著富集到抗逆相关路径中。KEGG分析显示,生根启动慢的无性系中的DEGs富集到更多的生物路径中,核糖体相关基因仅显著富集在生根启动快的无性系中,而糖酵解/糖异生、次生代谢产物的生物合成和氧化磷酸化相关基因均显著富集在生根启动慢的无性系中。共有202个DEGs显著富集在植物激素信号转导路径中,且生长素信号转导路径中的生长素响应基因家族(AUX/IAA),细胞分裂素信号转导路径中的CRE1及赤霉素信号转导路径中的转录因子在两个无性系中的表达量呈显著差异。【结论】尾叶桉生根启动慢的和启动快的无性系在转移至生根培养基上之前及之后3个时期的基因表达变化均不同,具有相同表达模式的DEGs在生根启动慢的无性系和生根启动快的无
【Objective】This study was designed to examine differences in gene expression between Eucalyptus urophylla clones cultivated on the same medium and at different growth periods in order to provide genetic information for the analyses of differences in root development.【Method】We used two E.urophylla clones grown in the same rooting medium with contrasting differences in rooting initiation but with the same generation of subcultures.We performed transcriptome sequencing analysis using 2 mm length stem tissue from the base of each clone on 0,1,4 and 6 d when apparent morphological differences occurred in clone base following fast rooting initiation.We further identified the differentially expressed genes(DEGs)that caused rooting differences between clones using gene differential expression analysis,cluster analysis,GO analysis and KEGG analysis.【Result】A total of 20287 DEGs were obtained,which were significantly grouped into four subclusters.Among them,the number of DEGs with an increasing expression pattern in fast rooting initiation clone was much higher than that in slow rooting initiation clone,and the number of DEGs with less expression change between 1 and 0 d in slow rooting initiation clone were much higher than those in fast rooting initiation clone.After being transferred to IBA medium,the GO analysis showed that DEGs of subcluster 17 were significantly enriched in cell cycle⁃related pathways in the fast rooting initiation clone,while significantly enriched in the stress resistance⁃related pathways in the slow rooting initiation clone.The KEGG analysis showed that DEGs in the slow rooting initiation clone were enriched in more biological pathways,while DEGs involved in ribosome were only significantly enriched in the fast rooting initiation clone.DEGs involved in glycolysis,gluconeogenesis,secondary metabolites biosynthesis and oxidative phosphorylation were significantly enriched in the slow rooting initiation clone.A total of 202 DEGs were significantly enriched in the plant hormone signal
作者
廖焕琴
杨会肖
徐放
潘文
张卫华
陈新宇
朱报著
徐斌
王裕霞
杨晓慧
LIAO Huanqin;YANG Huixiao;XU Fang;PAN Wen;ZHANG Weihua;CHEN Xinyu;ZHU Baozhu;XU Bin;WANG Yuxia;YANG Xiaohui(Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangdong Academy of Forestry,Guangdong 510520,China)
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2023年第4期114-122,共9页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
广东省林业科技创新项目(2016KJCX002,2019KJCX003)。
