摘要
【目的】考察冬荪栽培不同土壤环境覆土层的理化性质、土壤微生物群落结构及其多样性,分析冬荪生长状态与覆土层细菌和真菌群落结构及组成的关系,为冬荪高效栽培提供理论依据。【方法】以林下壤土、耕地壤土和耕地沙质土冬荪覆土层土壤为材料,提取土壤总DNA,利用Illumina MiSeq测序平台进行高通量测序,分析不同土壤环境细菌和真菌物种多样性及群落组成,并结合土壤营养进行相关性分析,探讨土壤微生物多样性变化情况。【结果】冬荪菌丝、菌蕾及子实体生长期内,土壤全氮、全磷、碱解氮、速效钾、有效磷、有机质明显增加,pH呈持续下降趋势。不同土壤环境细菌多样性高于真菌,林地壤土细菌多样性低于耕地,而真菌多样性不显著。优势细菌门为变形菌门、放线菌门、酸杆菌门,优势真菌门为子囊菌门、担子菌门、被孢霉门;林地壤土的优势细菌属为热酸菌属、Archaeorhizomyces和慢生根瘤菌属,耕地壤土和耕地沙质土优势细菌为Vicinamibacterales。全磷、全钾、速效钾与鬼笔属物种丰度呈正相关,但不显著。【结论】冬荪大田栽培的覆土层全磷、全钾、速效钾与鬼笔属物种丰度变化呈正相关,生长期内pH呈下降趋势,林地壤土覆土层热酸菌属、Archaeorhizomyces、慢生根瘤菌为优势属,与土壤营养成分及含量相关,林地沙质土镰刀菌属为优势属,与作物连作相关。
[Objective]To reveal the relationship between the growth state of Phallus dongsun and its bacterial and fungal community structure in overlaying soil,the physicochemical properties,soil microbial community structure,diversity and other characteristic changes of P.dongsun in overlaying soil were investigated,which also provided theoretical basis for high-efficiency cultivation for P.dongsun.[Method]In this study,the P.dongsun in different soil environments(forest loam,cultivated land,cultivated sandy soil)was used as the material,then the total DNA was extracted,and the Illumina MiSeq sequencing plaform was used for high-throughput sequencing to analyze the spe-cies diversity and community composition of bacteria and fungi in different soil environments.Correlation analysis was carried out with soil nu-trient indicators to explore changes in soil microbial diversity.[Result]During the growth period of mycelium,button and fruiting bodies of P.dongsun,the total nitrogen,total phosphorus,alkali-hydrolysed nitrogen,available potassium,available phosphorus and organic matter in soil increased significantly,and pH showed a continuous decreasing trend.The bacterial diversity of different soil environments was higher than that of fungi,and the bacterial diversity of forest loam was lower than that of cultivated land,while the fungal diversity was not signifi-cant.The dominant bacterial phyla were Proteobacteria,Actinobacteria and Acidobacteria,and the dominant fungal phyla were Ascomycota,Basidiomycota and Mortierellomycota.The dominant bacterial genera in forest loam were Acidothermus,Archaeorhizomyces and Bradyrhizobi-um,while Vicinamibacterales were the dominant bacteria in cultivated loam and cultivated sandy soils.Total phosphorus,total potassium and available potassium were positively correlated with the abundance of Phallus species,but were not significant.[Conclusion]Total phosphor-us,total potassium and available potassium in overlaying soil of P.dongsun are positively correlated with the abundance of Phallus,whi
作者
王万坤
康超
曾维军
郑旋
杨玲
刘忠玄
王芳
王晶
WANG Wan-kun;KANG Chao;ZENG Wei-jun;ZHENG Xuan;YANG Ling;LIU Zhong-xuan;WANG Fang;WANG Jing(Guizhou Institute of Biology,Guiyang 550009,China)
出处
《西南农业学报》
CSCD
北大核心
2023年第6期1222-1233,共12页
Southwest China Journal of Agricultural Sciences
基金
贵州省科技计划项目[黔科合支撑(2022)一般114]
贵州省科技计划项目[黔科合支撑(2020)1Y067]。
