摘要
【目的】探明银白杨根际土壤微生物群落对盐碱胁迫的响应,为进一步通过植物与微生物的互作实现盐渍土壤生态修复提供依据。【方法】以不同浓度(2%、3%、4%、5%)NaHCO_(3)、Na_(2)CO_(3)、NaCl溶液对银白杨幼苗进行胁迫处理,采用高通量测序技术对银白杨幼苗根际土壤微生物群落组成、多样性及标志物进行分析。【结果】种植银白杨与种植其他物种盐碱地根际土壤微生物群落分类学组成无明显区别,不同盐浓度胁迫条件下,银白杨根际微生物的群落多样性差异不显著;相比于低浓度(2%)胁迫,3种盐溶液在高浓度(4%)胁迫条件下均有相当数量的ASV(amplicon sequence variants),相对丰度显著上调,其中,NaCl胁迫的差异ASV最少;NaHCO_(3)胁迫下银白杨根际土壤微生物中属水平上的标志物为小双孢菌属(Microbispora)、金黄线菌属(Chryseolinea)、Saccharimonadales、东氏菌属(Dongia)和独岛氏菌属(Dokdonella),Na_(2)CO_(3)胁迫下的标志物为列契瓦尼尔氏菌属(Lechevalieria)、红细菌属(Rhodobacter)、豆形囊菌属(Phaselicystis)、粉色微菌属(Roseimicrobium)、苯基杆菌属(Phenylobacterium)、假水黏细菌属(Pseudenhygromyxa)、BIyi10,NaCl胁迫下的标志物为斯克尔曼氏菌属(Skermanella)和假红游动菌属(Pseudorhodoplanes)。【结论】相对于植被地上部分,根际土壤微生物群落受盐碱胁迫影响较小,不同盐碱及浓度下物种多样性差异不显著,盐碱胁迫下部分ASV上调,并初步筛选8个属的标志微生物,分别为2%Na_(2)CO_(3)胁迫的列契瓦尼尔氏菌属(Lechevalieria)、红细菌属(Rhodobacter)、豆形囊菌属(Phaselicystis)、粉色微菌属(Roseimicrobium),3%Na_(2)CO_(3)胁迫的苯基杆菌属(Phenylobacterium),4%Na_(2)CO_(3)胁迫的假水黏细菌属(Pseudenhygromyxa)、BIyi10和1个尚未归类的属。
【Objective】The response of microbial community around rhizosphere soil of Populus alba to saline-alkali stress was discussed to provide the scientific basis for realizing ecological remediation of salt-affected soil by means of the interaction between plants and microbes.【Method】The composition,diversity and landmark of microbial community around rhizosphere soil of P.alba seedlings treated with different concentrations(2%,3%,4%and 5%)of NaHCO 3,Na 2 CO 3 and NaCl were analyzed by the high throughput sequencing.【Result】There was no obvious difference in taxonomic composition of rhizosphere soil microbial community between P.alba and other plant species.There was no significant difference in community diversity of P.alba rhizosphere soil microbes under the stress of different salt concentrations.The relative abundance of ASV(amplicon sequence variants)increased under the stress of high concentration(4%)of three salts compared with 2%concentration stress of three salts.The difference in ASV was the least under NaCl stress.The landmarks at genus level of P.alba rhizosphere soil microbes under NaHCO 3 stress were Microbispora,Chryseolinea,Saccharimonadales,Dongia and Dokdonella.The landmarks at genus level of P.alba rhizosphere soil microbes under Na 2 CO 3 stress were Lechevalieria,Rhodobacter,Phaselicystis,Roseimicrobium,Phenylobacterium,Pseudenhygromyxa and BIyi10.The landmarks at genus level of P.alba rhizosphere soil microbes under NaCl stress were Skermanella and Pseudorhodoplanes.【Conclusion】The saline-alkali stress had the less effect on rhizosphere soil microflora compared with the aboveground part of vegetation.There was no significant difference in species diversity under the stress of different salt types with different concentrations.Some ASV increased under saline-alkali stress.8 preliminarily screened mark microbes at genus level were Lechevalieria,Rhodobacter,Phaselicystis and Roseimicrobium stressed by 2%Na 2 CO 3,Phenylobacterium stressed by 3%Na 2 CO 3,Pseudenhygromyxa,BIyi10 and a
作者
李丹
蒋泽廷
李琳
王琪瑶
LI Dan;JIANG Zeting;LI Lin;WANG Qiyao(Heilongjiang Academy of Forestry Sciences,Harbin,Heilongjiang 150081;Northeast Forestry University,Harbin,Heilongjiang 150040;Key Laboratory of Forest Ecology&Forestry Ecological Engineering,Harbin,Heilongjiang 150081,China)
出处
《贵州农业科学》
CAS
2023年第8期76-84,共9页
Guizhou Agricultural Sciences
基金
黑龙江省自然基金联合引导项目(LH2020C102)
黑龙江省属科研院所科研业务费支持项目(CZKYF2021-2-C028)。
关键词
微生物群落
胁迫
根际土壤
银白杨
高通量测序
多样性
标志物
microbial community
stress
rhizosphere soil
Populus alba
high-throughput sequencing
diversity
landmark
