摘要
目的探讨慢性前列腺炎(CP)病人趋化因子、白细胞(WBC)、卵磷脂小体(SPL)、神经生长因子(NGF)的表达水平及其临床意义。方法根据美国国立卫生研究院的分类标准,收集2020年2月—2021年5月就诊的93例Ⅲ型CP病人,其中ⅢA型44例,ⅢB型49例,另外选择50例健康体检者作为对照组。采用CP症状指数评分表(CPSI)评估病人临床症状,采用酶联免疫吸附法检测前列腺液(EPS)中趋化因子单核细胞趋化因子(MCP-1)、巨噬细胞炎症因子α(MIP-1α)和NGF水平,EPS镜检WBC及SPL计数,分析不同分型病人EPS中趋化因子、WBC、SPL和NGF水平差异。结果ⅢA型和ⅢB型病人的WBC计数等级和SPL计数差异有统计学意义(Z=-9.053、-3.056,P<0.05)。ⅢA型和ⅢB型病人WBC、SPL计数与CPSI各项评分无相关性(P>0.05)。与对照组相比,ⅢA型和ⅢB型病人EPS中趋化因子MCP-1、MIP-1α及NGF水平均升高(F=101.397~196.707,P<0.05);与ⅢA型相比,ⅢB型病人趋化因子MCP-1、MIP-1α水平降低(P<0.05),NGF水平及CPSI总分升高(P<0.05)。CP病人趋化因子MCP-1、MIP-1α与CPSI量表中疼痛和不适评分存在正相关(r=0.405、0.427,P<0.05)。结论Ⅲ型CP病人EPS中趋化因子及NGF均高于对照组。ⅢA型与ⅢB型EPS镜检WBC及SPL计数存在差异,WBC和SPL可以用来对CP进行分型,但与CPSI症状评分无相关性。MCP-1和MIP-1α可用来评估CP病人的分型和严重程度。
Objective To investigate the expression levels of chemokines,white blood cell(WBC)count,small particles of lecithin(SPL),and nerve growth factor(NGF)and their clinical significance in patients with chronic prostatitis(CP).Me-thods According to the classification criteria established by the National Institutes of Health,93 patients with typeⅢCP who attended the hospital from February 2020 to May 2021 were enrolled,among whom 44 patients had typeⅢA CP and 49 had typeⅢB CP.A total of 50 individuals who underwent physical examination were enrolled as control group.Chronic Prostatitis Symptoms Index(CPSI)was used to evaluate the clinical symptoms of patients,ELISA was used to measure the levels of chemokines[monocyte chemoattractant protein-1(MCP-1)and macrophage inflammatory protein-1 alpha(MIP-1α)]and NGF in expressed prostatic secretion(EPS),and EPS microscopy was used to measure WBC and SPL count.The levels of chemokines,WBC,SPL,and NGF were compared between the patients with different types of CP.Results There were significant differences in WBC and SPL count between the patients with typeⅢA CP and those with typeⅢB CP(Z=-9.053,-3.056;P<0.05).There was no correlation between WBC/SPL count and CPSI scores in the patients with typeⅢA/ⅢB CP(P>0.05).Compared with the control group,the patients with typeⅢA/ⅢB CP had significant increases in the levels of the chemokines MCP-1 and MIP-1αand NGF in EPS(F=101.397-196.707,P<0.05).Compared with the patients with typeⅢA CP,the patients with typeⅢB CP had significant reductions in the levels of the chemokines MCP-1 and MIP-1α(P<0.05)and significant increases in the level of NGF and the total score of CPSI(P<0.05).The correlation analysis showed that in the patients with CP,the chemokines MCP-1 and MIP-1αwere positively correlated with the scores of pain and discomfort in CPSI scale(r=0.405,0.427;P<0.05).Conclusion The patients with typeⅢCP have higher levels of chemokines and NGF in EPS than the control group,and there are significant diffe-rences
作者
姜涵中
孙立江
JIANG Hanzhong;SUN Lijiang(Department of Urology,The Affiliated Hospital of Qingdao Univer-sity,Qingdao 266003,China)
出处
《青岛大学学报（医学版）》
CAS
2023年第3期397-400,共4页
Journal of Qingdao University(Medical Sciences)
基金
国家自然科学基金资助项目(81101932)。
