摘要
为了探究大豆抵抗大豆花叶病毒(Soybean mosaic virus,SMV)侵染的分子调控机制,挖掘抗病相关基因,培育抗病新品种,本研究从清除H2O2前后大豆响应SMV的转录组数据库中筛选得到1个差异表达的大豆病程相关蛋白基因GmPR1-6(Glyma.15G062400.1),利用生物信息学分析并结合实时荧光定量(Real time quantitative,RT-qPCR)技术以及病毒介导的基因沉默(Virus induced gene silencing,VIGS)等技术验证其在大豆抵抗SMV侵染过程中的功能。结果表明,GmPR1-6在非亲组合中转录水平的表达量显著高于亲和组合;GmPR1-6基因沉默植株叶片接种SMV的部位胼胝质积累面积增大、胼胝质荧光强度减弱,病毒在胞间扩散的能力增强,说明GmPR1-6基因在大豆抵抗SMV侵染过程中发挥正调控作用。研究结果为进一步探究病程相关蛋白在大豆抵抗SMV侵染过程中的功能提供试验依据。
It is crucial to investigate the molecular regulatory mechanisms of disease resistance to soybean mosaic virus SMV.It is of great importance for cultivation of disease-resistant soybean varieties to identify genes associated with disease resistance.In this study,a differentially expressed pathogenesis-related proteins gene,GmPR1-6(Glyma.15G062400.1),was screened out from the transcriptome database of soybean treated with H2O2 elimination in response to SMV infection. Analysis and verify the function of The function of GmPR1-6 in soybean resistance to SMV was analyzed using bioinformatics methods, real time quantitative PCR (RT-qPCR) and virus-mediated gene silencing (Virus induced gene silencing, VIGS). The results showed that GmPR1-6 expression was significantly higher in the incompatible combination compared to that in the compatible combination. The area of callose deposition around the SMV inoculation sites was significantly increased and the intensity of callose fluorescence was attenuated in the GmPR1-6 silenced plants after SMV inoculation. Moreover, the intercellular transport of SMV was also enhanced after silencing of the gene, indicating a potential role of GmPR1-6 in plant immunity. The results is a foundation for further exploring the function of pathogenesis related proteins in soybean in resistance to SMV.
作者
苏伟华
赵志华
齐梦楠
孙天杰
王冬梅
张洁
SU Weihua;ZHAO Zhihua;QI Mengnan;SUN Tianjie;WANG Dongmei;ZHANG Jie(State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology/College of Life Sciences,Hebei Agriculture University,Baoding 071001,China)
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2023年第4期8-15,共8页
Journal of Hebei Agricultural University
基金
河北省自然科学基金(C2020204132,C2020301020)
河北省现代农业产业技术体系创新团队建设大豆产业创新团队(326-0702-JSNTKSF)
河北省科技厅现代种业科技创新专项子课题(21326313D-1).
