摘要
目的 姜黄Curcumalonga主根形成中药姜黄,侧根形成药材郁金。为探知姜黄主根与侧根形成过程的差异和侧根生长膨大的分子机制,对姜黄主根与侧根进行转录组测序,挖掘促使侧根生长膨大的关键基因。方法 以姜黄主根和侧根为材料,采用Illumina HiSeq高通量测序平台进行转录组测序,组装与注释后进行差异表达基因筛选。结果 转录组测序共获得42.69 Gb clean data,共得到42 748条Unigene,其中35 456条被注释。主根和侧根中的差异表达基因(differentially expressed genes,DEGs)有1551个,基因本体(geneontology,GO)富集结果表明,姜黄主根与侧根的DEGs主要富集于代谢过程、细胞过程、催化过程、转运过程、结合等功能,COG富集结果表明,主根与侧根中DEGs主要富集于碳水化合物代谢和信号转导机制。KEGG富集分析显示,根中DEGs主要富集在淀粉和糖代谢、植物激素信号传递等代谢通路中。通过注释信息筛选出姜黄侧根生长膨大相关的差异表达基因,关键差异表达基因与通路分析表明侧根中蔗糖合成酶基因、生长素响应基因(SAUR32、SAUR76、SAUR50)的表达上调可能是姜黄侧根生长膨大的影响因素。结论 通过对姜黄主根与侧根高通量转录组测序,揭示了姜黄侧根生长膨大的关键基因,可以为姜黄根系生长的生物学机制提供参考。
Objective The main roots of Curcuma longa form Chinese medicine Jianghuang(Curcumae Longae Rhizoma),and the lateral roots form Yujin(Curcumae Radix).Transcriptome sequencing was conducted to explore the differences in the formation process of main roots and lateral roots of C.longa and the molecular mechanism of growth and expansion of ateral roots,so as to explore the key genes promoting lateral root growth and expansion.Methods The main roots and lateral roots of C.longa were used as materials,and transcriptome sequencing was performed by Illumina Hi Seq high-throughput sequencing platform.After assembly and annotation,differentially expressed genes were screened.Results A total of 42.69 Gb Clean Data and 42 748 Unigenes were obtained by transcriptome sequencing,of which 35 456 were annotated.There were 1551 differentially expressed genes(DEGs) in the main roots and lateral roots.The GO enrichment results showed that the DEGs in the main and lateral roots of C.longa were mainly enriched in metabolic processes,cellular processes,catalytic processes,transport processes,binding and other functions,and the COG enrichment results showed that the DEGs in the main and lateral roots were mainly enriched in carbohydrate metabolism and signal transduction mechanisms.KEGG enrichment analysis showed that DEGs were mainly enriched in starch and sugar metabolism and plant hormone signaling pathways.The differentially expressed genes related to lateral root development were screened from the annotated information.The key differentially expressed genes and pathway analysis indicated that the up-regulation of sucrose synthase gene and auxin response genes(SAUR32,SAUR76,SAUR50) in the lateral roots of C.longa may be the influencing factors of the lateral root growth expansion of C.longa.
作者
李永宁
尹彦棚
周罗静
蓝鑫
侯飞侠
彭成
高继海
LI Yong-ning;YIN Yan-peng;ZHOU Luo-jing;LAN Xin;HOU Fei-xia;PENG Cheng;GAO Ji-hai(State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)
出处
《中草药》
CAS
CSCD
北大核心
2023年第14期4623-4630,共8页
Chinese Traditional and Herbal Drugs
基金
西南特色中药资源多维评价多学科交叉创新团队(ZYYCXTD-D-202209)
中药品质性形成分子机制研究四川省青年科技创新研究团队(22CXTD0009)
川产地道药材大品种精深加工关键技术及产品开发的研究与示范(2020YFN0152)
杏林学者(QNXZ2018017,QNXZ2019001)。
关键词
姜黄
转录组
主根
侧根
生长
代谢过程
细胞过程
催化过程
转运过程
Curcuma longa L.
transcriptome
main root
lateral root
growth
metabolic processes
cellular processes
catalytic processes
transportprocesses
