摘要
目的 观察鹰嘴豆芽素A(BCA)对结肠癌细胞增殖迁移的影响,并探讨可能机制。方法 取对数期人结肠癌HCT-116细胞,随机分为对照组、BCA组、SB216763[Wnt/β-连环蛋白(β-catenin)通路激活剂]组、联合组。对照组不做处理,BCA组加入BCA(40μmol/L),SB216763组中加入SB216763(20μmol/L),联合组加入BCA(40μmol/L)+SB216763(20μmol/L)。MTT法检测各组细胞增殖能力;划痕实验检测各组细胞迁移能力;血管拟态形成实验检测各组细胞血管新生能力;免疫印迹法检测各组细胞兔抗人糖原合成酶激酶3β(GSK-3β)、β-catenin蛋白表达量。结果 与对照组比较,BCA组24、48、72 h MTT实验吸光度值、迁移率、β-catenin蛋白表达量降低,血管数量/视野减少,GSK-3β蛋白表达量升高(P<0.05),SB216763组24、48、72 h MTT实验吸光度值、迁移率、β-catenin蛋白表达量升高,血管数量/视野增加,GSK-3β蛋白表达量降低(P<0.05);与BCA组比较,联合组24、48、72 h MTT实验吸光度值、迁移率、β-catenin蛋白表达量升高,血管数量/视野增加,GSK-3β蛋白表达量降低(P<0.05);与SB216763组比较,联合组24、48、72 h MTT实验吸光度值、迁移率、β-catenin蛋白表达量降低,血管数量/视野减少,GSK-3β蛋白表达量升高(P<0.05)。结论 BCA可抑制结肠癌细胞增殖、迁移及血管新生,抑制Wnt/β-catenin信号通路可能是其作用机制之一。
Objective To observe the effect of biochanin A(BCA) on the proliferation and migration of colon cancer cells,and to explore the possible mechanism.Methods Log-phase human colon cancer HCT-116 cells were randomly divided into the control group,BCA group,SB216763 [Wnt/β-catenin pathway activator] group,and the combination group.The control group was left untreated,BCA(40 μmol/L) was added to the BCA group,SB21676(20 μmol/L) was added to the SB216763 group,and BCA(40 μmol/L) + SB216763(20 μmol/L) was added to the combination group.MTT method was used to detect cell proliferation ability in each group.The scratch test was used to detect the migration ability of each group of cells.Vascular mimicry formation experiment was used to detect the angiogenesis ability of each group of cells.Western blotting was used to detect the expression of rabbit anti-human glycogen synthase kinase 3β(GSK-3β) and β-catenin protein in each group of cells.Results Compared with the control group,24,48,72 h MTT experiment Absorbance values and mobility,β-catenin protein expression were decreased,the number of blood vessels/field of vision was decreased,and GSK-3β protein expression was increased in the BCA group(P<0.05),24,48,72 h MTT experiment Absorbance values,mobility,β-catenin protein expression were increased,the number of blood vessels/field of view was increased,and GSK-3β protein expression was decreased in the SB216763 group(P<0.05).Compared with the BCA group,24,48,72 h MTT experiment Absorbance values,mobility,β-catenin protein expression were increased,the number of blood vessels/field of view was increased,and GSK-3β protein expression was decreased in the combined group(P<0.05).Compared with the SB216763 group,24,48,72 h MTT experiment Absorbance values and mobility,β-catenin protein expression were decreased,the number of blood vessels/field of vision was decreased,and GSK-3β protein expression was increased in the combined group(P<0.05).Conclusion BCA can inhibit the proliferation,migration and angiogenesis
作者
陈晓燕
王莹莹
罗金键
CHEN Xiaoyan;WANG Yingying;LUO Jinjian(Zhengzhou Central Hospital Affiliated to Zhengzhou University,Zhengzhou,450000)
出处
《实用癌症杂志》
2023年第8期1219-1223,共5页
The Practical Journal of Cancer
