摘要
目的初步研究孕期四溴联苯醚(2,2′,4,4′-tetrabraomodiphenylether,BDE-47)暴露对母体和仔代小鼠脂肪因子、胰岛素敏感性的影响,并探讨其可能机制。方法雌性C57BL/6小鼠随机分为高、中、低剂量组和对照组,每组5只。适应性饲养1周后与同批健康成年雄鼠合笼,合笼成功后对雌鼠行10 d的BDE-47灌胃,灌胃1周后对母鼠行腹腔内注射葡萄糖耐量实验。仔鼠出生后6周时,所有小鼠禁食过夜后处死。酶联免疫吸附实验(enzyme-linked immuno sorbent assay,ELISA)测定母鼠血清胰岛素、瘦素和脂联素水平;实时荧光定量聚合酶链式反应(quantitative real-time PCR,qRT-PCR)和蛋白质印迹法分别检测母鼠和仔代小鼠肝组织中过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptorγ,PPARγ)、瘦素和脂联素的mRNA与蛋白表达水平。结果BDE-47暴露使母鼠葡萄糖代谢紊乱,仔鼠出生后早期发育期间体质量增加,染毒组母鼠及雄性仔鼠肝脏重量及脏器系数较对照组均增大(均P<0.05)。与对照组相比,高剂量组母鼠和雌性仔鼠肝组织中PPARγ的mRNA和蛋白表达降低,高剂量组雄性仔鼠肝组织中PPARγ的m RNA和蛋白表达升高(均P<0.05);高、中剂量组母鼠瘦素的mRNA和蛋白表达较对照组降低,高剂量组雌性仔鼠瘦素的m RNA表达较其他各组升高,染毒组雄性仔鼠肝组织中瘦素的mRNA表达较对照组降低(均P<0.05);中剂量组母鼠和雌性仔鼠脂联素的mRNA表达较对照组降低,各剂量组雄性仔鼠肝组织中脂联素的mRNA表达均较对照组降低(均P<0.05)。与雌性仔鼠比较,高剂量组雄性仔鼠的肝脏脏器系数增加、PPARγ的mRNA和蛋白表达升高,中、高剂量组雄性仔鼠瘦素蛋白表达较雌性仔鼠升高,低、高剂量组雄性仔鼠脂联素mRNA的表达较雌性仔鼠降低(P<0.05)。结论BDE-47使母鼠糖代谢紊乱,使仔代小鼠早期发育期间体质量增加,影响小鼠PPARγ、瘦素和�
Objective To preliminarily investigate the effects of 2,2',4,4'-tetrabromodiphenyl ether(BDE-47)exposure during pregnancy on adipokines and insulin sensitivity in maternal and offspring mice,explore its possible mechanisms.Methods The female C57BL/6 mice were randomly divided into high,medium,low dose groups and control group,five animals in each group.After being fed for one week,they were caged with healthy adult male mice purchased in the same batch.The female mice were given BDE-47 by intragastric administration for 10 days after successful cage closure.One week after gavage,the maternal mice were treated with glucose tolerance test by intraperitoneal injection.At six weeks after birth,all mice fasted overnight and were killed.The levels of serum insulin,leptin and adiponectin in maternal mice were measured by enzyme-linked immuno sorbent assay(ELISA).The mRNA and protein expression levels of peroxisome proliferator-activated receptor(PPAR)leptin and adiponectin in the liver tissues of maternal and offspring mice were detected by quantitative real-time PCR(qRT-PCR)and Western blot.Results BDE-47 exposure caused a disturbance of glucose metabolism in maternal mice.The body mass of the offspring mice increased during the early development period after birth,and the liver weight and organ coefficients of the maternal mice and male offspring in the exposure groups were higher than those in the control group(all P<0.05).Compared with the control group,the mRNA and protein expression of PPAR in the liver tissue of maternal mice and female offspring in the highdose group decreased,and the mRNA and protein expression of PPARy in the liver tissue of male offspring in the high dose group increased(all P<0.05).The mRNA and protein expression of leptin in maternal mice in the high and medium dose groups were lower than those of the control group,the mRNA expression of leptin in female offspring in the high dose group was higher than that of other groups,and the mRNA expression of leptin in the liver tissue of male offsri
作者
姜淑瑞
王嘉穗
王永治
王梦林
祖尼热·吐尔逊
刘早玲
JIANG Shu-rui;WANG Jia-sui;WANG Yong-zhi;WANG Meng-lin;Zunire·Tuerxun;LIU Zao-ling(School of Public Health,Xinjiang Medical University,Urumqi Xinjiang,830011,China)
出处
《职业与健康》
CAS
2023年第12期1589-1595,共7页
Occupation and Health
基金
国家自然科学基金(82160605,81760596)。
