摘要
以采自广西青龙山自然保护区的雅致栓孔菌菌株为试验材料,进行生物学特性的单因素试验与正交试验,探究了其驯化栽培的最适条件,并测定了栽培子实体水提物抗氧化活性。结果表明,雅致栓孔菌菌丝在以葡萄糖为碳源、蛋白胨为氮源、pH为5.5、温度为30℃的条件下生长最佳。4个因素对雅致栓孔菌菌丝生长的影响程度为:碳源>温度>氮源>pH。驯化栽培以棉籽壳作为栽培主料,搭配不同比例木屑进行栽培,结果表明,配方3(棉籽壳30%,木屑48%,麸皮20%,石灰1%,石膏1%)的菌丝生长速度达到10.46mm/d,平均产量54.74g/袋,生物学效率达到7.69%,为最佳栽培配方。采用超声法提取子实体水提物,子实体水提物浓度为2.5mg/mL时,ABTS的清除率达到100%,子实体水提物浓度为5mg/mL时,DPPH的清除率达到90.07%,表明该菌具有较高的抗氧化活性。
Single-factor tests and orthogonal tests were conducted on the biological characteristics of the strain of Trametes elegans collected from Qinglong Mountain Nature Reserve in Guangxi.The optimum conditions for its domestication and cultivation were studied,and the antioxidant activities of the aqueous extracts of the cultivated basidiomata were determined.The results showed using glucose as carbon source and peptone as nitrogen source,the mycelia grew well at pH 5.5 and incubation temperature of 30°C.The effects of the four factors on the mycelial growth of Trametes elegans were ranked as follows:carbon source>temperature>nitrogen source>pH.Cottonseed hulls were used as the main material for cultivation,with additional different proportions of wood chips.The results showed that formulation 3(cottonseed hulls 30%,wood chips 48%,bran 20%,lime 1%,gypsum 1%)was the best cultivation formulation,with mycelial growth rate reaching 10.46 mm/d,average yield of 54.74 g/bag and biological efficiency reaching 7.69%.Ultrasonication was used to extract the fruiting body water extract of T.elegans.The water extract concentration of 2.5 mg/mL showed 100%clearance of ABTS and 90.07%clearance of DPPH at a water extract concentration of 5 mg/mL,indicating that the fungus has high antioxidant activity.
作者
李苗苗
林建添
黄在兴
梁云龙
黄福常
刘斌
LI Miaomiao;LIN Jiantian;HUANG Zaixing;LIANG Yunlong;HUANG Fuchang;LIU Bin(Institute of Applied Microbiology,College of Agriculture,Institute of Edible Fungi,Guangxi University,Nanning 530005,Guangxi,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2023年第7期1506-1516,共11页
Mycosystema
基金
国家自然科学基金(31960006)。
关键词
雅致栓孔菌
单因素
正交试验
生物学效率
抗氧化活性
Trametes elegans
single factor
orthogonal test
biological efficiency
antioxidant activity
