摘要
目的探究低氧/冷暴露对肥胖大鼠模型白色脂肪棕色化的影响。方法通过高脂喂食构建肥胖大鼠模型并随机分为对照组、低氧组、低温组、低温低氧组,每组10只,观察各组大鼠体质量、体脂的变化,利用HE染色观察大鼠脂肪组织细胞形态变化并比较脂肪细胞面积,利用免疫荧光法、实时荧光定量PCR法及Western blot检测各组大鼠脂肪组织中过氧化物酶体增殖剂激活受体γ2(PPAR-γ2)、PR结构域结合因子16(PRDM16)和解偶联蛋白1(UCP-1)基因的表达及UCP-1蛋白的表达。结果与对照组相比,低氧组、低温组、低温低氧组大鼠体质量、体脂均降低,且低温低氧组大鼠体质量、体脂低于低氧组、低温组(P<0.05)。与对照组相比,低氧组、低温组、低温低氧组大鼠脂肪细胞面积减小,且低温低氧组大鼠脂肪细胞面积低于低氧组、低温组(P<0.05)。与对照组相比,低氧组、低温组、低温低氧组大鼠肩胛棕色脂肪组织PPAR-γ2、PRDM16、UCP-1基因表达均升高(F=378.495、102.061、322.443,P<0.05),肾周白色脂肪组织PPAR-γ2基因表达均降低(F=4.555,P<0.05),PRDM16、UCP-1基因表达均升高(F=24.387、163.660,P<0.05)。与对照组相比,低氧组、低温组、低温低氧组大鼠肩胛棕色脂肪组织与肾周白色脂肪组织UCP-1蛋白表达均升高(P<0.05);与低温低氧组相比,低氧组、低温组大鼠肩胛棕色脂肪组织与肾周白色脂肪组织UCP-1蛋白表达均更低(P<0.05)。结论低氧/冷暴露可通过调控脂肪内PPAR-γ2、PRDM16通路使UCP-1高表达,诱导白色脂肪棕色化并影响大鼠体质量。
Objective To investigate the effect of hypoxia/cold exposure on white fat browning in an obese rat model.Methods Obese rat model was constructed by high-fat feeding and randomly divided into control group,hypoxic group,low-temperature group and low-temperature hypoxic group,with 10 rats in each group.The changes of body weight and body fat were observed,and the morphological changes of rat adipose tissue cells were observed using HE staining and compared with the area of adipocytes.The expression of peroxisome proliferator-activated receptor 2(PPAR-γ2),PR structural domain binding factor 16(PRDM16)and uncoupling protein 1(UCP-1)gene and the expression of UCP-1 protein in the adipose tissue of rats were examined by immunofluorescence,realtime fluorescence quantitative PCR and Western blot.Results Compared with the control group,the body weight and body fat of rats in the hypoxic,hypothermic and cryogenic hypoxic groups were reduced,and the body weight and body fat of rats in the hypothermic hypoxic group were lower than those in the hypoxic and cryogenic groups(P<0.05).Compared with the control group,the adipocyte area of rats in the hypoxia,hypothermia and low-temperature hypoxia groups was reduced,and the adipocyte area of rats in the low-temperature hypoxia group was lower than that in the hypoxia and low-temperature groups(P<0.05).Compared with the control group,the expression of PPAR-γ2,PRDM16 and UCP-1 genes all increased in the scapular brown adipose tissue(F=378.495,102.061,322.443,P<0.05)and decreased in the perirenal white adipose tissue(F=4.555,P<0.05)in the hypoxic,hypothermic and low-temperature hypoxic groups of rats,PRDM16 and UCP-1 gene expression all increased(F=24.387,163.660,P<0.05).Compared with the control group,the expression of UCP-1 protein in scapular brown adipose tissue and perirenal white adipose tissue of rats in the hypoxic,hypothermic and low-temperature hypoxic groups increased(P<0.05);UCP-1 protein expression was lower in scapular brown adipose tissue and perirenal white adipose
作者
郜琨
杨历新
王叶
许海琦
Gao Kun;Yang Lixin;Wang Ye;Xu Haiqi(Dept of Endocrinology,Qinghai Provincial People's Hospital,Xining 810007)
出处
《安徽医科大学学报》
CAS
北大核心
2023年第8期1274-1279,共6页
Acta Universitatis Medicinalis Anhui
基金
青海省科技项目(编号:2016-ZJ-707)。
