摘要
【背景】本团队前期研究发现芽孢杆菌DU-106具有良好的健康益处,但都是基于活菌的研究,对灭活菌的研究尚未开展。【目的】探究芽孢杆菌DU-106裂解物对特应性皮炎的治疗效果及作用机制。【方法】以2,4-二硝基氟苯诱导的特应性皮炎BALB/c小鼠为模型,通过病理学观察、免疫组化等手段研究其治疗效果,并测定NF-κB通路中基因与蛋白表达量,揭示其内在机制。【结果】芽孢杆菌DU-106裂解物有效缓解了特应性皮炎小鼠的病理学特征,减轻了肥大细胞的浸润,降低了免疫球蛋白E、肿瘤坏死因子-α(TNF-α)和干扰素-γ的含量(P<0.05)。芽孢杆菌DU-106裂解物可能是通过抑制NF-κB信号通路中TNF-α、IKKα和NF-κB基因表达水平和蛋白磷酸化而实现抗特应性皮炎效果。【结论】芽孢杆菌DU-106裂解物可作为一种后生元,对特应性皮炎具有积极的治疗作用。
[Background]Our research team have discovered that Bacillus sp.DU-106 has good health benefits in previous studies.However,the conclusion is based on the studies of living bacteria and the studies of inactivated bacteria remain to be carried out.[Objective]To investigate the effect and mechanism of Bacillus sp.DU-106 lysate in the treatment of atopic dermatitis.[Methods]Pathological observation and immunohistochemistry were employed to study the therapeutic effect of Bacillus sp.DU-106 lysate on the mouse model of 2,4-dinitrofluorobenzene-induced atopic dermatitis.The expression levels of the proteins involved in the nuclear factor kappa-B(NF-κB)signaling pathway were determined to reveal the mechanism.[Results]Bacillus sp.DU-106 lysate alleviated the pathological changes,reduced the infiltration of mast cells,and lowered the levels of immunoglobulin E,tumor necrosis factor-α(TNF-α),and interferon-γ(P<0.05)in the model mice.Furthermore,Bacillus sp.DU-106 lysate down-regulated the expression levels and inhibited the phosphorylation of TNF-α,inhibitory kappa B kinaseα(IKKα),and NF-κB in the NF-κB signaling pathway in the model mice.[Conclusion]Bacillus sp.DU-106 lysate can be used as a probiotic with therapeutic effects on atopic dermatitis.
作者
赵文俊
张余威
李伟杰
赵超凡
李彦力
杜冰
黎攀
ZHAO Wenjun;ZHANG Yuwei;LI Weijie;ZHAO Chaofan;LI Yanli;DU Bing;LI Pan(College of Food Science,South China Agricultural University,Guangzhou 510642,Guangdong,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2023年第7期2970-2982,共13页
Microbiology China
基金
广东省重点领域研发计划(2020B020226008)
国家现代农业产业技术体系(CARS-21)。
