摘要
目的:观察Beagle犬脂肪干细胞在向成骨细胞分化过程中富血小板纤维蛋白(Platelet-richfibrin,PRF)渗出液的作用,及其与支架材料纳米羟基磷灰石支架生物相容性的实验研究。方法:酶消化法获得Beagle犬脂肪干细胞,将收集的富血小板纤维蛋白渗出液分为成骨诱导矿化液组(含成骨矿化液+5%PRF渗出液)、B组对照组(含成骨矿化液的10%FBS的DMEM)和C组空白组(普通10%FBS的DMEM培养液),观察PRF渗出液对成骨的影响,并用环境扫描电镜观察Beagle犬脂肪干细胞与经PRF渗出液孵育的纳米羟基磷灰石支架复合培养后的附着增殖情况。结果:在PRF渗出液的作用下,脂肪干细胞能够更加有效地向成骨细胞分化,形成更多的钙化结节,结节细小致密。环境扫描电镜观察,脂肪干细胞能在支架上充分地附着伸展。结论:PRF渗出液能够有效促进脂肪干细胞向成骨细胞分化,纳米羟基磷灰石三维支架材料具有很好的生物相容性。
Objective To investigate the effect ofplatelet-rich fibrin extract(PRFe)on the diff entiation of Beagleadiposederi vedstemcellsintoosteoblast,and to study the biocompatibility with nano-hydroxyapatite scaff olds.Methods Beagle ADSCs were obtained by enzyme digestion method.Platelette-rich fibrin exudate collected were divided into osteogenic induced mineralization solution group(containing osteogenic mineralization solution+5%PRFe),control group B(DMEM containing 10% FBS of osteogenic mineralization solution)and blank group C(DMEM medium of normal 10%FBS).The effect of PRF exudate on osteogenesis was observed,and the attachment proliferation of Beagle dog adipose stem cells and nano-hydroxyapatite scaff old incubated by PRFe were observed by environmental scanning electron microscopy.Results Under the action of PRF exudate,adipose stem cells can differentiate more efficiently into osteoblasts,more calcifi ed nodules form,and the nodules are small and dense.Environmental scanning electron microscope observation,adipose stem cells can be fully attached and extended on the scaff old.Conclusion PRFe can eff ectively promote the differentiation of adipose stem cells into osteoblasts,and nano-hydroxyapatite three-dimensional scaff old material has good biocompatibility.
作者
李志鹏
叶眉
李峥
吴刚
张晨
王丽雯
陈辰
LI Zhipeng;YE Mei;LI Zheng;WU Gang;ZHANG Chen;WANG Liwen;CHEN Chen(Department of Stomatology,Anhui No.2 Provincial People's Hospital,Hefei 230041,Anhui,China;Department of Clinical Medicine,Shanghai Smartee Denti-Technology Co.,Ltd.,Shanghai 201203,China;Department of Stomatology,Anhui Medical College,Hefei 230601,Anhui,China;Department of Pharmacy,the First Affi liated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230036,Anhui,China)
出处
《中国美容医学》
CAS
2023年第7期74-77,共4页
Chinese Journal of Aesthetic Medicine
基金
安徽省高等学校自然科学一般项目(编号:12925KJ2018B10)。
