摘要
目的:探讨红景天苷(SAL)对心肌梗死小鼠死亡率、心功能和心肌重构的影响。方法:小鼠随机分成假手术(Sham)组、心肌梗死未治疗(MI)组和SAL组,每组小鼠20只,通过结扎小鼠冠状动脉前降支建立小鼠心肌梗死模型,建模后SAL组给予SAL灌胃,MI组和Sham组给予生理盐水灌胃,21后检测各组小鼠的死亡率、心脏功能、心肌纤维化程度和梗死面积及炎症因子表达水平等指标。结果:Sham组、SAL组和MI组的存活率分别为100%、80%和50%(P<0.05)。与MI组相比,SAL组的心脏质量与体质量比值、肺质量与体质量比值、左室舒张末期内径和左室收缩末期内径明显降低,左室射血分数和左室短轴收缩率明显升高,梗死面积和瘢痕厚度明显减小,肿瘤坏死因子(TNF)-α、转化生长因子(TGF)-β1、白细胞介素(IL)-1β、凋亡信号调节激酶1(ASK1)等表达水平显著降低,Bcl-2、血管内皮生长因子(VEGF)、磷酸化蛋白激酶B(p-Akt)、磷酸化内皮一氧化氮合酶(p-eNOS)的表达水平显著升高(P均<0.05)。结论:SAL能够减轻小鼠心肌梗死后的死亡率,改善心功能,降低心肌纤维化,促进血管新生,减小梗死面积并改善心肌重构。
Objective:To investigate whether salidroside(SAL)therapy could reduce mortality,improve cardiac function and alleviate myocardial remodeling in mice with myocardial infarction.Methods:The mice were divided into Sham group,SAL group and myocardial infarction(MI)group.The mouse model of MI was established by ligating anterior descending branch of the coronary artery.Then mice in SAL group were treated with SAL intragastric administration and mice in Sham and MI group were treated with saline.After 21 days,the mortality,heart function,degree of myocardialfibrosis,infarct size and expression of inflammatory factor in each group was determined.Results:The survival rate was 100%,80%and 50%in Sham,SAL and MI group,respectively(P<0.05).Compared with MI group,mice in SAL group had impoved heart function,degree of fibrosis,infarct size,apoptosis index and angiogenesis.In addition,the expression levels of tumor necrosis factor-α,transforming growth factor-β1,interleukin-1βand apoptosis signal-regulated kinase 1 were decreased(P<0.05),whereas the expression levels of BCL-2,vascular endothelial growth factor,p-Akt and p-eNOS were increased in SAL group(P<0.05).Conclusion:This study suggests that salidroside could reduce mortality,myocardialfibrosis,infarct size and improve myocardial function and remodeling after myocardial infarction in mice.
作者
刘佳
武澎
陈彭生
LIU Jia;WU Peng;CHEN Pengsheng(Department of Clinical Laboratory,Xuzhou Central Hospital,Xuzhou 221009;Department of Cardiology,The First Affiliated Hospital of Nanjing Medical University,Nanjing 210029;Department of Cardiology,Xuzhou Central Hospital,Xuzhou 221009,China)
出处
《国际心血管病杂志》
2023年第4期229-234,241,共7页
International Journal of Cardiovascular Disease
基金
国家自然科学基金(82000335)
徐州市科技项目(KC17125)。
关键词
红景天苷
心肌梗死
心肌重构
Salidroside
Myocardial infarction
Myocardial remodeling
