摘要
目的在羅氏Cobas c501全自動生化分析儀上確立檢測血清總膽酸的常見干擾值:溶血指數(Hemolysis index,H)、黃疸指數(Icterus index,I)、脂血指數(Lipemia index,L)。方法設立最大允許干擾值dmax為0.67 umol/L,用希森美康(SYSMEX)專用干擾檢查A試劑盒,參考EP7-A2文件要求,進行干擾物實驗,對干擾物濃度及干擾程度之間的關係進行分析,並確立各干擾值。結果溶血干擾:為正干擾,回歸方程y=0.135x+5.509,相關係數R2=0.999;黃疸干擾:為正干擾,回歸方程y=0.069x+5.413,相關係數R2=0.998;脂血干擾:為正干擾,回歸方程y=0.063x+6.273,相關係數R2=0.992。結論在羅氏Cobas c501全自動生化分析儀上,檢測血清總膽酸,當H≦10、I≦20、L≦15時,所產生的干擾值≦dmax,檢測的結果能符合臨床診斷、治療的要求。
Objective To establish common interference values for detecting serum total bile acid on the Roche Cobas c501 automatic biochemical analyzer:Hemolysis index(H),Icterus index(I),and Lipemia index(L).Methods The maximum allowable interference value dmax was set to 0.67μmol/L,the SYSMEX special interference check A kit was used,the requirements of EP7-A2 were referred to,interference experiments were conducted,and the difference between the concentration of interference and the degree of interference was determined.The relationship was analyzed,and the interference value was established.Results H Hemolysis interference:positive interference,regression equation y=0.135x+5.509,correlation coefficient R2=0.999;Icterus interference:positive interference,regression equation y=0.069x+5.413,correlation coefficient R2=0.998;lipemia interference:positive interference,regression equation y=0.063x+6.273,correlation coefficient R2=0.992.Conclusion On the Roche Cobas c501 automatic biochemical analyzer,when detecting serum total bile acid,when H≤10,I≤20,and L≤15,the interference value generated is≤dmax,and the detection result can meet the clinical diagnosis and treatment claim.
作者
蕭子楓
周曉蘭
梁式賢
鄭巧儀
李素娟
SIU Chi Fong;ZHOU Xiao Lan;LEONG Sek In;CHEANG Hao I;LEI Sou Kun(Department of Clinical Laborator,Kiang Wu Hospital,Macao,china)
出处
《镜湖医学》
2023年第1期91-93,共3页
MEDICAL JOURNAL OF KIANG WU
关键词
總膽酸
干擾值
指數
生化分析儀
Total bile acid
interference value
index
biochemical analyzer
