摘要
目的对血液核酸检测拆分阳性率的影响因素进行分析,以便为今后更好地开展核酸检测工作提供参考依据。方法收集厦门地区2019年1月1日—2022年10月31日献血者标本进行6人份混合核酸检测。统计分析年份、检测者、试剂批号、仪器组合方式、混检反应性pool CT值、标本背景间的拆分阳性率。结果共完成核酸检测标本234715份,其中混检反应性pool 428个,拆分反应性pool 248个,拆分阳性率57.9%。年份、检测者、试剂批号、仪器组合、标本背景对拆分阳性率无影响(P>0.05);混检反应性pool不同CT值组间的拆分阳性率比较有差异(χ^(2)=69.587,P<0.05)。2022年有2个月份拆分阳性率明显异常,经过及时处理后恢复正常。结论混检反应性pool的CT值高低是影响本地区拆分阳性率的主要因素。应当每月进行拆分阳性率监测,一旦出现明显变化,需要及时进行综合分析和处理,确保血液检测质量。
Objective To analyze the influencing factors of the repeat reactive(RR)rates of minipools implicated in minipool(MP)nucleic acid testing(NAT)in Xiamen Blood Center,in order to provide reference for NAT.Methods Samples of blood donors from January 1,2019 to October 31,2022 were collected in Xiamen Blood Center and tested by MP-NAT(pools of six).Statistical analysis and comparison of MP-NAT RR rates was performed among different years,testers,reagent batches,instrument combinations,CT values of MP-NAT reactive pools and sample backgrounds.Results A total of 234715 blood samples were tested by MP-NAT,and 428 pools were reactive,in which 248 pools were individual-donor NAT reactive,with a MP-NAT RR rate of 57.9%.The difference of MP-NAT RR rates were not statistically significant among different years,testers,reagent batches,instrument combinations,and sample backgrounds(P0.05).The difference of MP-NAT RR rates among different CT values of MP-NAT reactive pools was statistically significant(χ~2=69.587,P0.05).Significantly abnormal RR rate accurred in two months in 2022,and returned to normal after timely handling.Conclusion The MP-NAT RR rates is one of the important indicators to monitor the quality of NAT.Once there is a significant change in the MP-NAT RR rates,comprehensive analysis and timely handling should be carried out to ensure the quality of blood detection.
作者
吴佳煌
庄淑程
裴斌
欧山海
WU Jiahuang;ZHUANG Shucheng;PEI Bin;OU Shanhai(Xiamen Blood Center,Xiamen 361004,China.)
出处
《中国输血杂志》
CAS
2023年第7期587-590,共4页
Chinese Journal of Blood Transfusion
基金
厦门市医疗卫生科技计划项目(3502Z20194100)。
关键词
核酸检测
拆分阳性率
非特异性扩增
混合检测
CT值
nucleic acid testing(NAT)
MP-NAT RR rates
nonspecific amplification
minipool
CT value
