摘要
近年来,二维(2D)金属-有机框架(MOF)纳米复合材料被广泛的应用于生物医学领域,尤其是在抗菌方面。在此,我们通过光照诱导还银离子成功在二维MOF纳米片上生长银纳米粒子,得到了一种银纳米粒子(AgNPs)修饰的二维ZrFc-MOF(MOF-Ag)纳米片,并将其用于光热增强Ag+释放抗菌治疗。通过水热法和超声处理合成MOF纳米片,然后通过原位光辐照诱导还原在MOF纳米片上生长AgNPs。系列表征结果表明AgNPs成功负载到MOF纳米片上。聚乙烯吡咯烷酮(PVP)的修饰不仅可以增强MOF-Ag在溶液中的稳定性,还可以增强它的生物相容性。在近红外激光(NIR)照射下,MOF纳米片可以在短时间升温,而温度的升高可以加速AgNPs在溶液中氧化为银离子。通过细菌生长曲线、菌落相对数和细菌形态变化等实验表明PVP@MOF-Ag纳米片具有优异的广谱杀菌性能。此外,2D MOF纳米片良好的光热性能不仅可以增强Ag+的释放,还可以增强细胞膜的通透性,随后进入细菌中的Ag+可以诱导内源性活性氧的产生,从而引发细菌的氧化应激,实现高效抗菌。基于良好的体外抗菌性能,进一步将PVP@MOF-Ag纳米片用于小鼠伤口愈合,在此期间PVP@MOF-Ag纳米片表现出良好的治疗效果和生物安全性。我们的研究结果表明,PVP@MOF-Ag纳米片可以作为光热增强Ag+释放抗菌治疗和伤口愈合的有效平台。
Recently,two-dimensional(2D)metal-organic framework(MOF)nanosheet-based composites have been extensively investigated as promising materials for biomedical applications,including antibacterial applications.This study reports the synthesis of silver nanoparticle(Ag NP)-modified 2D Zrferrocene-MOF(MOF-Ag)nanosheets by growing Ag NPs on 2D MOF nanosheets via light irradiation-induced reduction for photothermally enhanced silver ion(Ag+)release antibacterial treatment.The MOF nanosheets were synthesized by a hydrothermal method followed by ultrasonic treatment.Subsequently,Ag NPs were grown on the MOF nanosheets to obtain MOF-Ag nanosheets by in situ light irradiation-induced reduction.Various characterization results,including transmission electron microscopy(TEM),X-ray photoelectron spectroscopy(XPS),and inductively coupled plasma-mass spectrometry(ICP-MS),showed that Ag NPs were successfully loaded onto the surface of the MOF nanosheets.Fourier transform infrared(FTIR)spectroscopy further confirmed the successful modification of the MOF-Ag surface with polyvinylpyrrolidone(PVP).The modification with of PVP not only enhanced the stability of MOF-Ag in solution,but also enhanced its biocompatibility.Under 808 nm near-infrared laser(NIR)irradiation,the MOF nanosheets exhibited good photothermal properties and photothermal conversion efficiency.The temperature increase induced by the photothermal effect accelerates the oxidation of Ag NPs to Ag+,and thus MOF-Ag continuously releases silver ions to kill bacteria.It was concluded that PVP-functionalized MOF-Ag(PVP@MOF-Ag)nanosheets have good antibacterial properties using experimental analyses such as bacterial growth curves,relative number of colonies,and morphological changes of bacteria.PVP@MOF-Ag nanosheets not only kills S.aureus but also inhibits E.coli growth more efficiently,exhibiting broad-spectrum bactericidal properties.Additionally,the good photothermal performance of the 2D MOF nanosheets enhanced Ag+release and cell membrane permeability.Ag NPs release Ag
作者
赵信硕
邱海燕
邵依
王攀捷
余石龙
李海
周郁斌
周战
马录芳
谭超良
Xinshuo Zhao;Haiyan Qiu;Yi Shao;Panjie Wang;Shilong Yu;Hai Li;Yubin Zhou;Zhan Zhou;Lufang Ma;Chaoliang Tan(College of Chemistry and Chemical Engineering,Henan Key Laboratory of Function-Oriented Porous Materials,Luoyang Normal University,Luoyang 471934,Henan Province,China;The First Dongguan Affiliated Hospital,Guangdong Medical University,Dongguan 523808,Guangdong Province,China;Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs and School of Pharmacy,Guangdong Medical University,Dongguan 523808,Guangdong Province,China;College of Chemistry and Chemical Engineering,Henan Polytechnic University,Jiaozuo 454000,Henan Province,China;Institute of Advanced Materials,Key Laboratory of Flexible Electronics,Nanjing Tech University,Nanjing 211816,China;Department of Chemistry and Center of Super-Diamond and Advanced Films(COSDAF),City University of Hong Kong,Kowloon,Hong Kong SAR 999077,China;Shenzhen Research Institute,City University of Hong Kong,Shenzhen 518057,Guangdong Province,China)
出处
《物理化学学报》
SCIE
CAS
CSCD
北大核心
2023年第7期100-109,共10页
Acta Physico-Chimica Sinica
基金
国家自然科学基金(52102348,22171123,22005259)
河南省高校科技创新人才计划(23HASTIT016)
广东省基础与应用基础研究基金(2019A1515111112,2021A1515011831)
广东省普通高校重点领域专项(2021ZDZX2061)
香港城市大学启动基金(9610495)资助项目。
