摘要
基于表面增强拉曼光谱的磁免疫分析(简称SERS磁免疫)因其可实现样品的快速分离、富集、和无损检测以及具有灵敏度高、精准及快速等优点而备受关注。本文综述了SERS磁免疫分析基本原理及国内外的研究进展,并介绍了SERS磁免疫与微流控、侧向流动分析及荧光联用检测技术以解决其在微量快速检测、多组分分析及不同位点分析方面的困难;最后针对影响SERS磁免疫灵敏度提升的途径等进行展望。
Surface enhanced Raman spectroscopy(SERS)based magnetic immunoassay has attracted considerable attention due to its ability to achieve rapid separation,enrichment,and non-destructive detection of targets with high sensitivity,accuracy and efficiency.Herein,the principle of SERS based magnetic immunoassay and its practical applications were reviewed;SERS based magnetic immunoassay combined with microfluidic,lateral flow analysis,and fluorescence detection techniques was introduced to solve the difficulties in rapid detection of trace amounts,multi-component analysis and identification of different sites for overcoming the drawback in lacking the spatiotemporal resolution.Finally,several methods to improve the sensitivity and feasibility of SERS based magnetic immunoassay were prospected.
作者
张晨杰
姚建林
ZHANG Chenjie;YAO Jianlin(College of Chemistry,Chemical Engineering and Material Sciences,Soochow University,Suzhou 215123)
出处
《光散射学报》
2023年第2期83-96,共14页
The Journal of Light Scattering
基金
国家自然科学基金(No.22172109,21773166)。
关键词
SERS
磁免疫
联用技术
灵敏度
标记分子
SERS
Magnetic immunoassay
Combined detection technology
Sensitivity
labelled molecules
