摘要
基于西伯利亚白刺红果、黑果果皮转录组数据,本研究筛选并克隆获得一个与西伯利亚白刺果实花青素和原花青素合成代谢相关的R2R3-MYB转录因子,命名为NsMYB5。该基因ORF为840 bp,编码279个氨基酸,NsMYB5具有完整的SANT、MYB domain及HLH-MYB domain结构域,属于R2R3-MYB转录因子,与调控花青素和原花青素合成相关的香雪兰FhMYB5同源性较高。过表达NsMYB5烟草花瓣和雄蕊积累大量花青素,呈现深紫色,而叶片和茎仅有少量花青素积累,局部呈现淡紫色。转基因株系中花的花青素和原花青素含量均显著高于野生型(P<0.05)。结果表明,NsMYB5可以正向调控花青素和原花青素的合成,这为西伯利亚白刺果实果皮呈色的分子遗传机理提供了理论依据。
Based on transcriptome data from red and black fruit skins of Nitraria sibirica Pall.,a R2R3-MYB transcription factor regulating anthocyanin and proanthocyanidin metabolism in fruits of Nitraria sibirica Pall.was screened and cloned,which was named as NsMYB5.The ORF of NsMYB5was 840 bp,encoding 279 amino acids.NsMYB5had complete SANT,MYB domain and HLH-MYB domain,which belonged to R2R3-MYB transcription factor.NsMYB5had a higher homology with FhMYB5,which regulated both anthocyanin and proanthocyanin synthesis.The petals and stamens of transgenic tobacco lines accumulated a large amount of anthocyanin,which presented a dark purple color,while the leaves and stems only accumulated a small amount of anthocyanin,which presented a partly light purple color.The anthocyanidin and proanthocyanidin contents of transgenic line were significantly higher than those of wild type(P<0.05).The results indicated that NsMYB5can positively regulate the synthesis of anthocyanins and proanthocyanidin.This study provides a theoretical basis for the molecular genetic mechanism of black fruit peels of Nitraria sibirica Pall.
作者
包雪梅
宗渊
胡娜
刘宝龙
王洪伦
BAO Xuemei;ZONG Yuan;HU Na;LIU Baolong;WANG Honglun(Northwest Institute of Plateau Biology,Key Laboratory of Tibetan Medicine Research of Chinese Academy of Sciences,Xining 810008,China;Qinghai Normal University,Xining 810008,China;University of Chinese Academy of Sciences,Beijing 100049,China;Key Laboratory of Crop Molecular Breeding of Qinghai Province,Xining 810001,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2023年第8期1215-1222,共8页
Acta Agriculturae Boreali-occidentalis Sinica
基金
青海省科技厅项目(2021-ZJ-T05)。
