摘要
为阐明BMP/Smad信号通路下游的转录因子Smad9对鹅卵泡发育的调控作用,研究采用RNAi技术干扰Smad9基因在鹅卵泡颗粒细胞的表达。通过对SMAD9蛋白表达定位,Smad9基因干扰后颗粒细胞的增殖情况以及卵泡发育相关激素及其受体的表达分析,探讨Smad9基因表达对鹅卵泡颗粒细胞增殖及其内分泌功能的影响。结果显示,SMAD9蛋白在鹅卵泡仅表达于颗粒细胞,Smad9-siRNA显著抑制Smad9的表达。Smad9干扰后颗粒细胞的增殖能力明显降低,细胞上清中雌二醇(E 2)的水平显著下降,孕酮(P 4)水平未见明显变化,细胞色素P450芳香化酶基因(CYP19A1)和促黄体素受体(LHR)基因的表达显著下降,促卵泡素受体(FSHR)基因的表达无显著变化。这些结果表明,干扰Smad9基因表达对鹅卵泡颗粒细胞增殖和内分泌功能均产生显著影响,其机制可能是Smad9基因表达抑制后引起颗粒细胞E 2合成减少和LHR的表达下降有关。
In order to elucidate the regulatory effect of the transcription factor Smad9downstream of the BMP/Smad signaling pathway on goose follicle development,RNAi technology was used to interfere with the expression of Smad9gene in goose granulosa cells.Through the localization of SMAD9protein expression,the proliferation of granulosa cells and the expression analysis of follicle development-related hormones and their receptors after Smad9gene interference,the effect of Smad9gene expression on the proliferation and endocrine function of goose follicular granulosa cells was discussed.The results showed that SMAD9protein was only expressed in granulosa cells in goose follicles,and Smad9-siRNA significantly inhibited the expression of Smad9.After reproductive gene interference,the proliferation ability of granulosa cells significantly decreased,the level of estradiol(E 2)in cell supernatant significantly decreased,the level of progesterone(P 4)did not significantly changed,the expression of cytochrome P450 aromatase gene(CYP19A1)and luteinizing hormone receptor(LHR)gene significantly decreased,and the expression of follicle-stimulating hormone receptor(FSHR)gene did not significantly changed.These results showed that interference with Smad9gene expression significantly affects proliferation and endocrine function of goose follicle granulosa cells,and the mechanism may be releads to the reduction of E 2 synthesis and the decreased expression of LHR in granulosa cells after Smad9gene expression inhibition.
作者
余道伦
佘德勇
葛凯
杨磊
张瑞娜
左瑞华
胡文睿
YU Daolun;SHE Deyong;GE Kai;YANG Lei;ZHANG Ruina;ZUO Ruihua;HU Wenrui(College of Biological and Pharmaceutical Engineering,West Anhui University,Lu’an Anhui 237012,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2023年第8期1166-1172,共7页
Acta Agriculturae Boreali-occidentalis Sinica
基金
皖西学院高层次人才科研启动费(WGKQ2021031)
皖西学院自然科学研究重点项目(WXZR201934)
安徽省高等学校省级质量工程重点项目(2020jyxm2128)
安徽省大学生创新创业训练计划项目(S202010376114)。
