摘要
银离子(Ag^(+))是水体污染中最主要的有害重金属之一,由于其生物积累性和潜在毒性,Ag^(+)能够与酶/蛋白质结合并导致其失活.此外,生物硫醇,如谷胱甘肽(GSH)、半胱氨酸(Cys)在细胞生长、代谢和维持适当的氧化还原状态中发挥重要作用.因此,有效检测生物液体中的Ag^(+)和生物硫醇在生物化学和临床化学中具有重要意义.本工作以4-溴邻苯二胺和多巴胺为前体,通过水热法合成了氮溴共掺杂CDs(N,Br-CDs).N,Br-CDs具有强烈的红光,发射波长为640 nm,相对量子产率高达24.6%.再通过Ag^(+)猝灭其荧光,生物硫醇恢复其荧光,我们构建了一种荧光开关传感器用来连续检测Ag^(+)和生物硫醇,此方法已适用于人尿中生物硫醇的检测.本工作设计的荧光开关传感器用来连续检测Ag^(+)和生物硫醇有以下亮点:首先设计出红色荧光进行检测,这是目前极少被报道的;其次是相较于其他传感器,该传感器具有灵敏度极高、操作简单、线性范围较宽等优势.
Silver ion(Ag^(+))is one of the most important harmful heavy metals in water pollution.Due to its bioaccumulation and potential toxicity,Ag^(+)can bind to enzymes/proteins and cause their inactivation.In addition,biothiols,such as glutathione(GSH)and cysteine(Cys),play an important role in cell growth,metabolism and maintenance of an appropriate redox state.Therefore,the effective detection of Ag^(+)and biothiols in biological fluids is of great significance in biochemistry and clinical chemistry.In this work,N,Br-codoped CDs(N,Br-CDs)were synthesized by hydrothermal method using 4-bromo-o-phenylenediamine and dopamine as precursors.N,Br-CDs had a strong red emission wavelength of 640 nm and a relative quantum yield of 24.6%.Then,the fluorescence was quenched by Ag^(+)and restored by biothiols.We constructed a fluorescent switch sensor for continuous detection of Ag^(+)and biothiols.This method has been applied to the detection of biothiols in human urine.
作者
雷甜甜
陈宽
宋功武
何瑜
LEI Tiantian;CHEN Kuan;SONG Gongwu;HE Yu(College of Chemistry and Chemical Engineering,Hubei University,Wuhan 430062,China)
出处
《湖北大学学报（自然科学版）》
CAS
2023年第4期574-580,共7页
Journal of Hubei University：Natural Science
基金
国家自然科学基金(21707030)资助。
