摘要
油菜素内酯(BR)是一类植物甾醇类激素,对植物生长发育及胁迫应答具有显著的调控作用,马铃薯中StCYP85A3作为BR合成酶的编码基因,功能仍待进一步探究。本研究从‘川芋10号’马铃薯中克隆出该基因,生物信息学分析表明该蛋白具有催化BR合成的典型亲氧结合域,属于CYP85As家族成员。通过组织表达分析发现该基因在块茎芽眼及根中表达量最高,且芽眼中的表达量随着储藏时间的延长而增加,在块茎芽眼休眠解除时表达迅速升高。同时发现外源BR可诱导马铃薯芽眼及根中StCYP85A3表达量升高,从而显著促进了种薯萌芽及幼苗根的伸长。通过在拟南芥野生型及cyp85a2突变体中过表达StCYP85A3发现,过表达株系种子萌发及根系伸长都早于野生型,同时回补株系弥补了突变体种子萌发、植株根系生长迟缓的缺陷。此外,外源BR对过表达株系种子萌发、根系伸长无显著促进作用,但显著促进了野生型、突变体及回补株系种子萌发、根系伸长。上述马铃薯及拟南芥试验结果表明, StCYP85A3具有促进萌芽及根系伸长的功能。
Brassinolide(BR),a kind of phytosterol hormone,plays the significant role in regulating plant growth and development and stress response.The function of StCYP85A3 as the encoding gene of BR synthease in potato remains to be further explored.In this study,the gene was cloned from potato‘Chuanyu 10’.Bioinformatics analysis showed that this protein had a typical aerobic binding domain for catalyzing BR synthesis and belonged to the CYP85As family.Tissue expression analysis indicated that the relative expression level of this gene was the highest in the bud eye of the tuber and the root,and the expression amount in the bud eye increased with the extension of storage time,and the expression increased rapidly when the dormancy of the tuber bud eye was released.At the same time,the exogenous BR could induce the relative expression level of StCYP85A3 in potato bud eye and root,which significantly promoted the germination of seed potato and the elongation of seedling root.The overexpression of StCYP85A3 in Arabidopsis wild-type and mutant cyp85a2 demonstrated that the seed germination and root elongation of the overexpression strains were earlier than wild type,and the defects of mutant seed germination and plant root growth retardation were remedied by supplementing strains.In addition,exogenous BR did not significantly promote seed germination and root elongation of over expressed strains,but significantly promoted seed germination and root elongation of wild type,mutant,and complementary strains.The above potato and Arabidopsis thaliana experiments showed that StCYP85A3 had the function of promoting germination and root elongation.
作者
刘洁
蔡诚诚
刘石锋
邓孟胜
王雪枫
温和
李罗品
严奉君
王西瑶
LIU Jie;CAI Cheng-Cheng;LIU Shi-Feng;DENG Meng-Sheng;WANG Xue-Feng;WEN He;LI Luo-Pin;YAN Feng-Jun;WANG Xi-Yao(State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;Potato Research and Development Center,College of Agriculture,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;College of Biological Engineering,Sichuan University of Light Chemical Industry,Yibin 644000,Sichuan,China)
出处
《作物学报》
CAS
CSCD
北大核心
2023年第9期2462-2471,共10页
Acta Agronomica Sinica
基金
马铃薯种薯活力调控的分子机制与应用研究项目(SKL-ZY202203)
国家现代农业技术体系四川薯类创新团队项目(sccxtd-2023-09)资助。
