摘要
目的探讨卡托普利对注意缺陷多动障碍(ADHD)模型大鼠行为学及神经递质水平的影响。方法取4周龄WKY大鼠及自发性高血压大鼠(SHR)分为阴性对照组(WKY组)、模型组(SHR组)、卡托普利组(CAP组),其中CAP组采用50 mg/kg卡托普利灌胃处理。4周后检测大鼠行为学(自发活动开场实验、高架十字迷宫、Morris水迷宫)及神经递质[多巴胺(DA)、去甲肾上腺素(NE)、5-羟色胺(5-HT)]水平。结果自发活动开场实验结果显示,SHR组和CAP组总路程、中央路程较WKY组增加,而CAP组总路程较SHR组减少,差异有统计学意义(P<0.05)。高架十字迷宫结果显示,SHR组开放臂进入次数比例及开放臂停留时间比例较WKY组增多,而CAP组较SHR组减少,差异有统计学意义(P<0.05)。Morris水迷宫结果显示,SHR组、CAP组穿越平台次数和目标象限时间较WKY组增多,且CAP组多于SHR组,差异有统计学意义(P<0.05)。SHR组DA水平较WKY组明显降低,而CAP组DA水平较SHR组明显增加,差异有统计学意义(P<0.05)。结论卡托普利可改善SHR大鼠ADHD症状。
Objective To study the effect of captopril on the behavioristics and neurotransmitter level in the model rat with attention deficit hyperactivity disorder(ADHD).Methods Four-week-old WKY rats and spontaneous hypertensive rats(SHR)were divided into the negative control group(WKY),model group(SHR)and captopril group(SHR+CAP).The captopril group was treated with 50 mg/kg captopril by gavage.The rat behavioriustics(spontaneous activity opening experiment,elevated cross maze,Morris water maze)and neurotransmitter levels[dopamine(DA),noradrenaline(NE)and 5-hydroxytryptamine(5-HT)]were detected after 4 weeks.Results The results of spontaneous activity opening experiment showed that the total distance and central distance in the SHR+CAP group were increased compared with the WKY group,while the total distance in the CAP group was decreased compared with the SHR group,and the differences were statistically significant(P<0.05).The elevated cross maze results showed that the proportion of open arm entry times and the proportion of open arm residence time in the SHR group were increased compared with those in the WKY group,while the CAP group was decreased compared with that in the SHR group,and the differences were statistically significant(P<0.05).The Moris water maze results showed that the number of crossing platform and target quadrant time in the SHR group and CAP group were increased compared with those in the WKY group,moreover the CAP group was more than the SHR group,and the differences was statistically significant(P<0.05).The DA level in the SHR group was significantly decreased compared with that in the WKY group,while the DA level in the CAP group was significantly increased compared with that in the SHR group,and the difference was statistically significant(P<0.05).Conclusion Captopril could improve the ADHD symptoms in SHR rats.
作者
白娟
徐梦月
李宏宝
BAI Juan;XU Mengyue;LI Hongbao(Department of Anesthesiology and Surgery/Center for Brain Science,First Affiliated Hospital of Xi’an Jiaotong University,Xi’an,Shaanxi 710061,China;Second Clinical College,Shaanxi University of Chinese Medicine,Xianyang,Shaanxi 712000,China;School of Basic Medical Sciences,Xi’an Jiaotong University,Xi’an,Shaanxi 710061,China)
出处
《重庆医学》
CAS
2023年第13期1926-1931,共6页
Chongqing medicine
基金
国家自然科学基金项目(82004083)
西安交通大学第一附属医院科研发展基金项目(2021ZXY-19)。
关键词
卡托普利
注意缺陷多动障碍
行为学
去甲肾上腺素
5-羟色胺
多巴胺
captopril
attention deficit hyperactivity disorder
behavioristics
noradrenaline
5-hydroxytryptamine
dopamine
