摘要
目的探究基因DACT3促进神经胶质瘤细胞(U251、U118)铁死亡的分子机制。方法培养人U251、U118胶质瘤细胞,慢病毒转染构建稳定过表达DACT3的细胞后,qRT-PCR和Western blot检测转染效率。上述细胞分成3组:Ctrl组(对照组)、DACT3组(过表达DACT3细胞组)和DACT3+ferrostatin-1组(过表达DACT3+铁死亡抑制剂)。采用CCK-8检测细胞生存率,铁离子、MDA、谷胱甘肽(glutatione,GSH)检测试剂盒测定细胞铁离子、MDA、GSH含量,Western blot检测GPX4、SLC7A11、TFR1表达。进一步将上述细胞分为以下3组:Ctrl组(对照组)、DACT3组(过表达DACT3细胞组)和DACT3+siBMP4组(过表达DACT3+敲低BMP4组),再次利用CCK-8检测细胞生存率,铁离子、MDA、GSH检测试剂盒测定细胞铁离子、MDA、GSH含量,Western blot检测GPX4、SLC7A11、TFR1、BMP4、p-Smad表达。结果DACT3组中DACT3 mRNA和蛋白表达均显著高于Ctrl组;与Ctrl组比较,DACT3组细胞生存率明显下降,铁离子、MDA含量显著升高,GSH含量明显下降,GPX4、SLC7A11表达显著下降,TFR1、p-Smad、BMP4表达明显升高,差异均具有统计学意义(P<0.05);DACT3+ferrostatin-1组、DACT3+siBMP4组均能一定程度抵消DACT3导致的上述指标的变化,差异具有统计学意义(P<0.05)。结论DACT3可以促进U251、U118胶质瘤细胞铁死亡,其机制与BMP4/SMAD信号通路激活有关。
Objective To investigate the molecular mechanism of DACT3 promoting ferroptosis in human glioma cell lines U251 and U118.Methods Glioma U251 and U118 cells were transfected with lentiviral vector carrying DACT3 to establish a cell line with stable over-expression of DACT3,which was verified for the efficiency of transfection by real-time PCR and Western blotting.Then the above cells were divided into control group(Ctrl group),DACT3 overexpression group(DACT3 group)and DACT3 overexpression+ferroptosis inhibitor group(DACT3+ferrostatin-1 group).CCK-8 assay was applied to detect the cell survival rate,and iron ion,malondialdehyde(MDA)and glutatione(GSH)reagent kits were employed to determine the contents of iron ion,MDA and GSH in the cells.Western blotting was applied to measure the protein levels of GPX4,SLC7A11,TFR1,BMP4,and p-Smad.The above cells were further divided into Ctrl group,DACT3 group and DACT3+siBMP4 group(knockdown of BMP4).Above experiments were performed again for cell survival rate,contents of iron ion,MDA and GSH,and protein levels of above proteins.Results The expression of DACT3 at mRNA and protein levels was significantly higher in the DACT3 group than the Ctrl group.The DACT3 group had significantly reduced cell survival rate,increased contents of iron ions and MDA but decreased content of GSH,down-regulation of GPX4 and SLC7A11 and up-regulation of TFR1,p-Smad and BMP4 when compared with the Ctrl group(P<0.05).However,ferrostatin-1 treatment and knockdown of BMP4 reversed the changes of the above indicators caused by DACT3 to a certain extent,with statistical significances(P<0.05).Conclusion DACT3 promotes the ferroptosis of glioma U251 and U118 cells,which may be associated with the activation of BMP4/Smad signaling pathway.
作者
郑立
邓枚
王利亚
张继勤
岳建和
韩国强
彭硕
刘健
尹浩
谭赢
ZHENG Li;DENG Mei;WANG Liya;ZHANG Jiqin;YUE Jianhe;HAN Guoqiang;PENG Shuo;LIU Jian;YIN Hao;TAN Ying(Graduate School of Zunyi Medical University,Zunyi,Guizhou Province,563000;Department of Neurosurgery,Guizhou Provincial People’s Hospital,Guiyang,Guizhou Province,550002,China;Department of Anesthesiology,Guizhou Provincial People’s Hospital,Guiyang,Guizhou Province,550002,China)
出处
《陆军军医大学学报》
CAS
CSCD
北大核心
2023年第13期1397-1404,共8页
Journal of Army Medical University
基金
国家自然科学基金地区科学基金(81960454,81960344)
贵州省科学技术厅科学技术基金(黔科合基础[2020]1Z066)。
