摘要
目的:通过对新生小鼠卵巢单个生殖细胞转录组数据进行挖掘,探究可变剪切事件是否参与调控小鼠卵泡组装过程的阶段转换。方法:基于小鼠单个生殖细胞转录组数据,用生物信息学方法分析卵泡组装过程3个细胞发育阶段的可变剪切事件及阶段转换之间的差异可变剪切事件,并用PCR验证差异可变剪切相关同源异构体的变化。结果:在小鼠卵泡组装3个发育阶段转换之间均检测到差异可变剪切事件,其中卵泡生成阶段可变剪切事件以及包囊破裂到卵泡生成时期的差异可变剪切事件富集最多,PCR验证了减数分裂相关同源异构体的变化。结论:可变剪切和小鼠卵泡组装细胞发育阶段转换相关,调控生殖细胞到卵母细胞转变过程。
Objective:To investigate whether alternative splicing regulate the stage transition of follicle assembly in mice,the transcriptome data of single germ cells in new born mouse ovary were analyzed.Methods:Bioinformatics analysis were performed on transcriptome data of single germ cells in mice to detect alternative splicing events in three cell development stages during follicle assembly and differential alternative splicing events between stage transitions.PCR was used to validate dynamic stage transition related isoform expression.Results:The current study found abundant differential alternative splicing events between stage transitions during follicle assembly in mice.The most enriched splicing events were detected in follicle stage,also the most enriched differential splicing events were detected between cyst break down and follicle stage.The results of PCR validated the presence of isoforms of meiosis related genes and showed their shifts of alternative isoform usage at stage transitions.Conclusion:Alternative splicing was associated with cell development stage transitions during follicle formation in mice,regulating the transformation from germ cell to oocytes.
作者
孙文亚
何元林
陈秋臻
李晶
SUN Wenya;HE Yuanlin;CHEN Qiuzhen;LI Jing(State Key Laboratory of Reproductive Medicine,Nanjing Medical University,Nanjing 211166,China)
出处
《南京医科大学学报（自然科学版）》
CAS
北大核心
2023年第7期893-899,共7页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家重点研发计划(2018YFC1004203)。
关键词
卵泡组装
可变剪切
单细胞转录组
follicle assembly
alternative splicing
single cell transcriptome
