摘要
目的 探讨党参脂溶性成分提取工艺,分析党参脂溶性成分。方法 采用单因素分析和均匀设计实验优化党参脂溶性成分提取温度、液料比、提取时间和超声功率4个参数后,利用乙醚超声辅助提取法对党参脂溶性成分进行提取,比较不同产地党参脂溶性成分含量,并分析巫山庙宇党参(简称庙党)脂溶性成分的变化。结果 (1)在提取时间为10~50 min时,随着时间的增加,脂溶性成分提取率呈升高趋势;当提取时间超过50 min后,提取率开始下降。(2)在提取温度为15~45℃时,随着提取温度的升高,脂溶性成分提取率呈升高趋势;当提取温度超过45℃后,提取率开始下降。(3)在料液比为1∶5~1∶10时,脂溶性成分提取率呈升高趋势;当料液比超过1∶10后,提取率开始降低。(4)在超声功率为80~120 W时,脂溶性成分提取率呈升高趋势;当超声功率达到120 W时,提取率达到最大值2.072 6%,随后提取率开始降低。10个不同产地党参脂溶性成分含量存在较大差异。在低温下(4℃)贮存1个月后,庙党脂溶性成分从原来的90种减少至29种,且成分种类发生了较大变化。结论 采用乙醚超声辅助提取法提取党参脂溶性成分具有工艺稳定、高效、可靠的特点,不同产地党参脂溶性成分含量差异明显,以庙党含量最高,部分解释了庙党气味浓烈的原因,同时应重视庙党脂溶性成分的储存条件和时间。
Objective To explore the extraction process of liposoluble constituents of Codonopsis pilosula and analyze the liposoluble constituents of Codonopsis pilosula.Methods After using single factor analysis and uniform design experimentation to optimize the four parameters of extraction temperature,liquid to material ratio,extraction time,and ultrasound power for the liposoluble constituents of Codonopsis pilosula,the ether ultrasound assisted extraction method was used to extract the liposoluble constituents of Codonopsis pilosula.The content of liposoluble constituents of Codonopsis pilosula from different producing regions was compared,and the changes in liposoluble constituents of Wushan Miaoyu Codonopsis pilosula(referred to as Miao Dang)were analyzed.Results(1)Within the extraction time range of 10-50 minutes,the extraction rate of liposoluble constituents showed an increasing trend with the increase of time.When the extraction time exceeds 50 minutes,the extraction rate began to decrease.(2)Within the extraction temperature range of 15-45℃,the extraction rate of liposoluble constituents showed an increasing trend with the increase of extraction temperature,When the extraction temperature exceeds 45℃,the extraction rate began to decrease.(3)Within the material liquid ratio range of 1∶5-1∶10,the extraction rate of liposoluble constituents showed an increasing trend.When the material liquid ratio exceeds 1∶10,the extraction rate began to decrease.(4)Within the ultrasound power range of 80-120 W,the extraction rate of liposoluble constituents showed an increasing trend.When the ultrasound power reached 120 W,the extraction rate reached the maximum value of 2.0726%,and then the extraction rate began to decrease.There were significantly differences in the content of liposoluble constituents in Codonopsis pilosula from 10 different places of production.After being stored at low temperature(4℃)for one month,the liposoluble constituents of Miao Dang decreased from 90 to 29,and the types of components underwe
作者
韩磊
陈前锋
祝慧凤
HAN Lei;CHEN Qianfeng;ZHU Huifeng(School of Pharmacy/School of Chinese Medicine,Southwest University,Chongqing 400715,China;Department of Internal Medicine,Southwest University Hospital,Chongqing 400715,China)
出处
《现代医药卫生》
2023年第13期2166-2173,2180,共9页
Journal of Modern Medicine & Health
基金
重庆市科卫联合中医药科技项目-重点项目(ZY201801002)
重庆市中医药重点建设学科西南大学康复学项目(2021-4322190044)
重庆市农委特色农业-产业链技术支撑项目(40802017)。
关键词
党参
脂溶性成分
乙醚超声辅助提取法
Codonopsis pilosula
Liposoluble constituents
Ether ultrasound assisted extraction method
