摘要
施用生物炭、根际促生菌是改良盐渍化土壤的重要手段,然而关于两者组合对盐渍化土壤性质及微生物群落组成的影响知之甚少。采用田间试验,设置不施肥(C0)、常规施肥(CK)、常规施肥+根际促生菌(PG)、常规施肥+生物炭(BC)及常规施肥+根际促生菌+生物炭(PGB)共5个处理,研究生物炭与根际促生菌处理对大棚种植番茄后盐渍化土壤理化性质、酶活性和微生物群落组成的影响。结果表明,与C0处理相比,施肥处理(CK、PG、BC、PGB)均可提高土壤有机质(OM)、全量养分(TN、TP、TK)、速效养分(AN、AP、AK)含量及土壤酶活性(NiR、UrE、CeL、LiP),降低土壤容重、总盐度和pH值,但CK处理效果不佳,各处理整体表现为CK<PG、BC<PGB。高通量测序表明,施用生物炭、根际促生菌均可显著增加土壤微生物群落多样性,促进鞘氨醇单胞菌属(Sphingomonas)、嗜盐单胞菌属(Halomonas)以及芽孢杆菌属(Bacillus)等有益菌群的增殖,且均以PGB处理的相对丰度最高。冗余分析(RDA)表明,OM是影响PGB处理微生物群落结构的关键环境因子。综上,在种植番茄的盐渍化土壤中施用生物炭或根际促生菌对土壤理化性质及土壤酶活具有一定影响,但以二者组合施用效果最佳,提高土壤有机质含量有助于发挥其效果。
Application of biochar(BC)and plant growth promoting rhizobacteria(PGPR)is an impor-tant means to improve saline soil,however,little is known about the effects of their combination on properties and microbial community composition of salinized soil.A field experiment was conducted by setting 5 treat-ments as no fertilization(C0),conventional fertilization(CK),conventional fertilization combined with PG-PR(PG),conventional fertilization combined with BC and conventional fertilization combined with PGPR and BC(PGB).The effects of the treatments on physicochemical properties,enzymes activities and microbial community composition of salinized soil after tomato planting in greenhouse were studied.The results showed that compared with C0 treatment,the fertilization treatments(CK,PG,BC,PGB)could increase soil organic matter(OM),total nutrients(TN,TP,TK),available nutrients(AN,AP,AK)contents and soil enzymes activities(NiR,UrE,CeL,LiP),and reduce soil bulk density,total salinity and pH value.The effect of CK was not satisfactory.The overall performance of those treatments showed as CK<PG and BC<PGB.The high-throughput sequencing results showed that the application of BC and PGPR could significantly increase soil mi-crobial community diversity and promote the proliferation of beneficial bacteria such as Sphingomonas,Halomonas and Bacillus,and PGB treatment had the highest relative abundance.Redundancy analysis(RDA)showed that OM was the key environmental factor affecting the microbial community structure of PGB treat-ment.In conclusion,the application of BC or PGPR in salinized soil planted with tomato had certain effects on soil physicochemical properties and enzyme activities,and their combination had the best effect;the increase of soil organic matter content would help to exert their effects.
作者
远兵强
田春丽
胡莹莹
Yuan Bingqiang;Tian Chunli;Hu Yingying(College of Agricultural Engineering,Henan Agricultural Professional College,Zhongmu 451450,China)
出处
《山东农业科学》
北大核心
2023年第6期101-109,共9页
Shandong Agricultural Sciences
基金
河南省重点研发与推广专项(21051039)
2019年河南省高等教育教学改革研究与实践重点项目(2019SJGLX642)
河南农业职业学院科研创新团队项目(HNACKT-2020-062)。
关键词
生物炭
根际促生菌
盐渍化土壤
微生物群落
土壤理化性质
酶活性
Biochar
Plant growth promoting rhizobacteria
Salinized soil
Microbial community
Soil physicochemical properties
Enzyme activities
