摘要
为了筛查动物医院临床血清样本中的干扰抗体以避免其实验室检测结果呈现假阳性,影响临床诊断,试验采用一种独立于物种的双抗体夹心酶联免疫吸附测定(ELISA)法对来自于福建省部分地区动物医院接诊的犬、猫血清样本进行干扰抗体筛选和评估,用鸡IgY替换纯化小鼠IgG进行干扰抗体消除试验,并对干扰抗体阳性率与样本基本特征进行关联性分析。结果表明:在83份犬血清样本中,有19份样本为干扰抗体阳性,阳性率为22.9%;在81份猫血清样本中,有11份样本为干扰抗体阳性,阳性率为13.6%。经干扰抗体消除试验后,所有阳性血清样本中的干扰抗体均得到了有效消除。不同年龄、性别、绝育情况与品种犬之间的干扰抗体阳性率均差异不显著(P>0.05);不同年龄猫之间的干扰抗体阳性率显著差异(P<0.05),不同性别、绝育情况与品种之间的干扰抗体阳性率差异不显著(P>0.05)。说明所检测的犬、猫血清样本中存在干扰抗体,用鸡IgY替换纯化小鼠IgG可对干扰抗体进行有效消除。
In order to screen the interfering antibodies in clinical serum samples from animal hospitals so as to avoid false positive results in laboratory tests and which may affect clinical diagnosis,in this experiment a species-independent double antibody sandwich ELISA was used to screen and evaluate interfering antibodies in serum samples from dogs and cats received from animal hospitals in some regions of Fujian Province.Chicken IgY was used to replace purified mouse IgG to carry out interference antibody elimination test,and the correlation between the positive rates of interference antibodies and the basic characteristics of the samples was analyzed.The results showed that among 83 dog serum samples,there were 19 samples positive for interference antibodies,with a positive rate of 22.9%.Among 81 cat serum samples,there were 11 samples positive for interference antibodies,with a positive rate of 13.6%.After the interference antibody elimination test,the interference antibodies in all positive serum samples were effectively eliminated.There was no significant difference in the positive rates of interference antibodies among dogs of different ages,genders,spaying and breeds(P>0.05).There was significant difference in the positive rates of interference antibodies among cats of different ages(P<0.05),but no significant difference was found in the positive rates of interference antibodies among cats of different genders,spaying and breeds(P>0.05).The results suggested that there were interfering antibodies in the detected dog and cat serum samples,and replacing purified mouse IgG with chicken IgY could effectively eliminate interfering antibodies.
作者
董波
艾欢
张晓东
章高强
钟晓威
魏兰
李成钰
林炜明
DONG Bo;AI Huan;ZHANG Xiaodong;ZHANG Gaoqiang;ZHONG Xiaowei;WEI Lan;LI Chengyu;LIN Weiming(College of Life Science,Longyan University,Longyan 364012,China;Fujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology,Longyan 364012,China;Fujian Provincial Higher Education Universities Key Laboratory of Preventive Veterinary Medicine and Biotechnology,Longyan 364012,China)
出处
《黑龙江畜牧兽医》
北大核心
2023年第12期87-91,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
中央引导地方科技发展专项(2018L3011)
福建省自然科学基金面上项目(2019J01804)
大学生创新训练项目(202111312030)。
