摘要
目的对2022年湖北省人和家养动物血清中荆门蜱病毒(JMTV)的抗体阳性率进行初步研究,评估荆门蜱病毒感染人群和动物的风险。方法将JMTV NS3蛋白在大肠埃希菌中表达并纯化,制备ELISA包被抗原,利用人和动物血清分别确定cut-off值;收集湖北省多个市牛羊养殖场采集的牛、羊血清,武汉市中心医院体检健康人血清,对这些血清样本以及实验室保存的林区居民血清进行JMTV特异性抗体以及核酸检测。结果初步建立基于NS3蛋白的JMTV抗体检测酶联免疫吸附试验(ELISA)。确定人血清、牛血清和羊血清的cut-off值分别为0.342、0.098和0.298。44份牛血清和193份羊血清中JMTV抗体阳性率分别为4.55%和3.63%。174份体检健康人和13份林区居民血清JMTV抗体结果均为阴性。所有样本中均未检测到JMTV的核酸片段。结论家养动物牛、羊血清中检出JMTV特异性抗体,提示需关注牛、羊作为蜱携带者导致病毒扩散的风险。
Objective To study the positive rate of Jingmen tick virus(JMTV)antibody in serum of domestic animals and assess the risk of JMTV infection in humans and domestic animals in Hubei province,2022.Methods JMTV NS3 protein as antigen was expressed in Escherichia coli and purified.ELISA envelope antigen was prepared,and cut-off values were determined by human and animal serum.Serum samples from cattle,sheep from farms and serum samples from healthy people from hospitals were collected in Hubei.JMTV-specific antibody and nucleic acid were detected in theses samples,as well as serum from forest residents stored in our laboratory.In addition,JMTV-specific nucleic acid was detected in all samples by RT-PCR method.Results An ELISA assay for JMTV antibody detection based on NS3 protein was established.The cut-off values of human,bovine and sheep serum were determined to be 0.342,0.098 and 0.298,respectively.The positive rates of JMTV antibody in 44 bovine and 193 sheep serum were 4.55%and 3.63%,respectively.JMTV-specific antibody were negative in 174 healthy subjects and 13 forest residents.No nucleic acid fragments of JMTV were detected in all samples.Conclusion JMTV specific antibody was detected in the serum of domestic cattle and sheep,suggesting that it is necessary to pay attention to the spread risk of the virus caused by cattle and sheep.
作者
张秀丹
李贝杰
王静
孙晖
郑雅匀
罗雪莲
Zhang Xiudan;Li Beijie;Wang Jing;Sun Hui;Zheng Yayun;Luo Xuelian(School of Public Health,Shanxi Medical University,Taiyuan 030001,Shanxi,China;National Institute for Communicable Disease Prevention and Control,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Department of Medical Laboratory,the Central Hospital of Wuhan,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430014,Hubei,China)
出处
《疾病监测》
CAS
CSCD
北大核心
2023年第5期537-542,共6页
Disease Surveillance
基金
国家自然科学基金(No.81802017)。
关键词
荆门蜱病毒
NS3
酶联免疫吸附试验
血清学
Jingmen tick virus
NS3
Enzyme-linked immunosorbent assay
Serological screening
