摘要
目的通过单因素和正交试验设计,对酸枣仁中5种活性成分的超声离心提取工艺进行优化。方法选择甲醇浓度、液固比、提取时间3个因素,采用单因素和正交表L 9(34)进行试验,以斯皮诺素、6-阿魏酰斯皮诺素、酸枣仁皂苷A、酸枣仁皂苷B和白桦脂酸的峰面积为指标,进行综合评价。结果酸枣仁最佳超声离心提取工艺为甲醇浓度80%、液固比7∶1(mL·g^(-1))、提取时间40 min。结论该提取工艺稳定可行,能够有效提取酸枣仁中活性成分,便于后续快速含量测定研究。
OBJECTIVE To optimize the ultrasonic centrifugal extraction process of five active components from Semen Ziziphi spinosae by single factor and orthogonal design.METHODS Three factors including methanol concentration,liquid-solid ratio and extraction time were selected,and the single factor and orthogonal table L 9(34)were used to carry out the test.The peak areas of spironocin,6-Feruloylspinosin,jujuboside A,juboside B and betulinic acid were used as indicators for comprehensive evaluation.RESULTS The best ultrasonic centrifugal extraction process of jujube kernel was methanol concentration 80%,liquid-solid ratio 7∶1(mL·g^(-1)),extraction time 40 min.CONCLUSION The extraction process is stable and feasible,which can extract the active components in Semen Ziziphi spinosae with great efficiency,and facilitate the subsequent rapid content determination.
作者
张琛武
成洁
甄会贤
杜学勤
郭宝林
张浩
ZHANG Chen-wu;CHENG Jie;ZHEN Hui-xian;DU Xue-qin;GUO Bao-lin;ZHANG Hao(ShanXi Pharmaceutical Vocational College,Taiyuan 030031,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Science,Peking Union Medical College,Beijing 100193,China;Huanggang Jingui Traditional Chinese Medicine Industry Development Co.,Ltd.,Huanggang 438000,China)
出处
《海峡药学》
2023年第5期35-40,共6页
Strait Pharmaceutical Journal
基金
山西药科职业学院教科研课题(项目编号:2021112)。
