摘要
目的探讨2型糖尿病(T2DM)大鼠血清中蛋白结合态铜(Cu)、锌(Zn)的水平。方法30只成年Sprague Dawley(SD)大鼠随机分为对照组(n=10,普通饲料)和T2DM模型组[n=20,高糖高脂饲料,腹腔注射链脲佐菌素(STZ)诱导造模],分别取2组大鼠心脏血离心检测血清中的Cu、Zn元素总形态及非蛋白结合形态;于0~300μg/L制作Cu、Zn元素的标准曲线,获取检定限和定量限;通过精密度实验,计算Cu、Zn总含量、非蛋白结合态含量及相对标准偏差(RSD);采用连续光源石墨炉原子吸收光谱法检测血清Cu、Zn总形态含量和非蛋白结合态含量,计算蛋白结合态含量。结果Cu、Zn元素在标准曲线范围内的相关系数(r)>0.999,RSD均<5%,加标回收率为87%~110%;与对照组相比,T2DM模型组大鼠血清中Cu总形态含量和蛋白结合态含量均升高(P<0.05),Zn总形态含量和蛋白结合态含量均降低(P<0.05),Cu、Zn总形态含量和蛋白结合态元素含量的比值均升高(P<0.05);大鼠血清Cu总形态含量和蛋白结合态含量与Zn总形态含量和蛋白结合态含量呈负相关关系(P<0.05)。结论T2DM大鼠血清中Cu、Zn总形态变化主要由蛋白结合态变化引起,测定2种元素的蛋白结合态含量较总形态含量可以更好地反映它们在DM过程中的生物学作用。
Objective To investigate the levels of protein-bound copper(Cu)and zinc(Zn)elements in serum of rats with type 2 diabetes mellitus(T2DM).Methods Thirty adult Sprague Dawley(SD)rats were randomly divided into control group(n=10,normal diet)and T2DM model group[n=20,high glucose and high fat diet,induced by peritoneal injection of streptozotocin(STZ)].The total morphology of Cu and Zn elements and non-protein binding morphology in the serum were detected by centrifugation in the heart blood of rats of 2 groups.The standard curves of Cu and Zn elements were prepared from 0-300μg/L,and the test and quantification limit were obtained.The total Cu,Zn content,non-protein bound state content and relative standard deviation(RSD)were calculated by precision experiments.Serum Cu and Zn total morphological content and non-protein bound state were measured by continuous light source,and the content of protein-bound state was calculated.Results The correlation coefficient(r)of Cu and Zn elements within the standard curve range were>0.999,and all the RSD were<5%.The spike recovery ranged from 87%to 110%.The recovery rate of the spike was 87%-110%.Compared with the control group,the total Cu and protein bound state in the serum of T2DM model group was increased(P<0.05),and the total Zn and protein bound state was decreased(P<0.05).The ratio of the total morphological content and the element content of Cu and Zn in the protein-bound state were all increased(P<0.05).Rat serum Cu total morphology content and protein bound state content as well as Zn total morphology content and protein bound state content were inversely correlated(P<0.05).Conclusion The total morphological changes of Cu and Zn in the serum of T2DM rats are mainly caused by the changes of protein-binding state.Testing the protein-binding state content of the two elements can better reflect their biological role in DM than the total morphological content.
作者
秦旭
李军
胡勇
于春
黄磊
娄波
罗茹丹
徐纬
QIN Xu;LI Jun;HU Yong;YU Chun;HUANG Lei;LOU Bo;LUO Rudan;XU Wei(School of Public Health&Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,Guiyang 550025,Guizhou,China)
出处
《贵州医科大学学报》
CAS
2023年第6期647-651,660,共6页
Journal of Guizhou Medical University
基金
教育部高等教育司创新训练项目(201910660020)
贵州省科技计划项目(黔科合LH字[2016]7378)
贵州省区域内一流学科建设项目-公共卫生与预防医学(黔教科研发[2017]85)。
关键词
糖尿病
2型
大鼠
血清
铜
锌
元素
蛋白结合态
含量分析
diabetes,type 2
rat
serum
copper
zinc
elements
protein bound state
content analysis
