摘要
目的:开发一种易于制备、具有良好生物氧化酶活性的铂纳米粒子探针,用于快速、选择性地检测Hg^(2+)。方法:利用硼氢化钠对氯铂酸进行还原后,快速加入N-乙酰-L-半胱氨酸(NAC)对其进行修饰,形成超小粒径的铂纳米粒子(PtNPs)。采用透射电子显微镜(TEM)、X射线光电子能谱(XPS)、红外光谱和zeta电位仪对乙酰半胱氨酸修饰的铂纳米粒子(NAC-PtNPs)的大小和形态、表面元素、表面基团以及带电荷情况进行了表征。利用3,3′,5,5′-四甲基联苯胺(TMB)对NAC-PtNPs的模拟生物酶活性进行了探索。通过Hg^(2+)对NAC-PtNPs的聚集抑制作用,将Hg^(2+)的含量与NAC-PtNPs的氧化酶活性联系起来,从而检测Hg^(2+)的含量。结果:平均粒径为3.26nm的NAC-PtNPs具有良好的稳定性和模拟生物氧化酶活性,Hg^(2+)的检测线性范围100nM至600nM,检测限为48.1nM。结论:超小粒径的NAC-PtNPs制备简单,且具有高稳定性、强氧化酶活性的特点。利用NAC-PtNPs与Hg^(2+)的相互作用,实现了对Hg^(2+)的选择性地检测。
Objective:To develop an easily prepared platinum nanoparticle probe with good oxidase activity for the rapid and selective detection of Hg^(2+).Methods:After reduction of chloroplatinic acid with sodium borohydride,it was modified by rapid addition of N-acetyl-L-cysteine(NAC)to form ultra-small size platinum nanoparticles(PtNPs).The size and morphology,surface elements,surface groups and charge of acetylcysteine-modified platinum nanoparticles(NAC-PtNPs)were characterized by transmission electron microscopy(TEM),X-ray photoelectron spectroscopy(XPS),infrared spectroscopy and Zeta potentiometer.3,3′,5,5′-tetramethylbenzidine(TMB)was used to investigate the activity of simulated biological enzymes of NAC-PtNPs.Through the aggregation inhibition effect of Hg^(2+)on NAC-PtNPs,the content of Hg^(2+)was related to the oxidase activity of NAC-PtNPs,so as to detect the content of Hg^(2+).Results:NAC-PtNPs with an average particle size of 3.26nm showed good stability and simulated oxidase activity.The linear range of Hg^(2+)detection was 100nM to 600nM,and the detection limit was 48.1 nm.Conclusions:The preparation of ultra-small particle size NAC-PtNPs is simple and has the characteristics of high stability and strong oxidase activity.The interaction between NAC-PtNPs and Hg^(2+)was used to selectively detect Hg^(2+).
作者
林晓昀
LIN Xiaoyun(Fujian Provincial Hospital,Fujian Fuzhou 350001,China)
出处
《北方药学》
2023年第4期5-9,共5页
Journal of North Pharmacy
基金
福建医科大学启航基金项目(No:2021QH1296).
