摘要
为探究葡萄锈果品质及果皮显微结构的成因,以‘阳光玫瑰’为试材,系统分析了成熟期间果锈发生状况与果实品质相关生理指标的变化,并采用石蜡切片、扫描电镜及透射电镜观察有无果锈果皮的显微结构。结果表明,有锈果的可溶性固形物显著高于无锈果,其可溶性糖、木质素和总酚含量均极显著高于无锈果,有锈果果皮和果肉的果糖、葡萄糖和总糖含量均极显著高于无锈果,有锈果果皮的没食子酸、绿原酸、表儿茶素、阿魏酸、槲皮苷和白藜芦醇含量极显著高于无锈果,分别高出110.40%、85.06%、88.01%、359.89%、396.73%和40.66%,其咖啡酸含量显著高于无锈果,增加了21.93%,且无锈果果皮中未检测出丁香酸和香豆酸。显微结构分析发现,有锈果果皮中无明显的角质层细胞,角质膜结构被破坏,表皮蜡质层相对稀疏,蜡质晶体大小不均一,排列紊乱;表皮细胞壁次生加厚,细胞膜结构和细胞器受到破坏。
To explore the cause of pericarp microstructure and fruit quality of russet fruit of grape,'Shine Muscat'grape was used as experimental material,The changes of fruit rust occurrence and physiological indexes related to fruit quality during ripening were systematically analyzed,and the paraffin section,scanning electron microscopy(sem)and transmission electron microscope were used to observe the microstructure of none russet fruit and russet fruit pericarp.The results showed that the soluble solid content of russet fruit was significantly higher than that of none russet fruit,and the contents of soluble sugar,lignin and total phenol in russet fruit were extremely signifi cantly higher than that of none russet fruit,and the contents of fructose,glucose and total sugar in russet grape pericarp and flesh were extremely significantly higher than those in none russet pericarp and flesh.The contents of gallic acid,chlorogenic acid,epicatechin,ferulic acid,quercetin and resveratrol in russet grape pericarp were extremely significantly higher than those in none russet pericarp,higher than 110.40%,85.06%,88.01%,359.89%,396.73%and 40.66%,respectively.The caffeic acid content in russet grape pericarp was significantly higher than that in none russet pericarp,increased by 21.93%.And the contents of syringic acid and coumaric acid in none russet pericarp were not detected.The microstructure analysis showed that there were no obvious cuticle cells in russet pericarp of'Shine Muscat'grape,the cuticle membrane structure was destroyed,the wax layer of pericarp epidermis was relatively sparse,the wax crystal size was uneven and disarranged,the cell wall of pericarp epidermis was thickened,the cell membrane structure and organelles were destroyed.
作者
黄艳
刘芳
黄科文
刘磊
梁东
王进
陈洪艳
吕秀兰
HUANG Yan;LIU Fang;HUANG Kewen;LIU Lei;LIANG Dong;WANG Jin;CHEN Hongyan;LYU Xiuan(Institute of Pomology and Olericulture,Sichuan Agricultural University,Chengdu 611130,China;Chengdu Academy of Agricultural and Forestry Sciences,Chengdu 611130,China;Leshan Normal University,Leshan 614000,China;Luding Agricultural Technology Extension and Soil Fertilizer Station,Luding 626100,China)
出处
《中外葡萄与葡萄酒》
北大核心
2023年第4期24-31,共8页
Sino-Overseas Grapevine & Wine
基金
成都市科技项目(2021-YF05-00076-SN)
四川省科普培训项目(2022JDKP0024)四川省重点研发项目(2022YFN0005)
葡萄资源四川省科技资源共享服务平台项目(2020JDPT0004)四川省现代农业产业技术体系创新团队专项资金
乐山师范学院校级学科建设重点科研项目(LZD028)。
关键词
阳光玫瑰
果锈
显微结构
果实品质
Shine Muscat
russet
microstructure
fruit quality
