摘要
目的 探讨LncRNA表型在急性痛风性关节炎(acute gouty arthritis,AGA)发病中的作用。方法 按病例纳入标准,研究选择AGA(湿热蕴结型)患者5例,予清热通络方配方颗粒治疗5 d;选择5例非痛风性关节炎健康人(healthy control,HC)不予任何处理;AGA服药后转为NAGA(non-acute gouty arthritis)组。采集AGA组、HC组、NAGA组的外周血单核细胞(PBMC)中LncRNA,利用Arraystar LncRNA Microarray芯片技术,观察3组LncRNA的表型差异。结果 和HC组比较,AGA组具有表型差异的LncRNA共有2 364条,其中1 174条表达上调,1 190条表达下调(Fold Change>2,P<0.05);和HC组比较,NAGA组具有表型差异的LncRNA共有802条,其中147条表达上调,655条表达下调(Fold Change>2,P<0.05);和AGA组比较,NAGA组具有表型差异的LncRNA共有171条,其中30条表达上调,141条表达下调(Fold Change>2,P<0.05);AGA组、NAGA组和HC组3组间有关联的LncRNA共有79条,其中68条LncRNA在AGA组表达上调、NAGA组表达下调,11条LncRNA在AGA组表达下调、在NAGA组表达上调。结论 LncRNA的表型差异可能和痛风的急性发作有关。
Objective To investigate the role of LncRNA phenotype in the pathogenesis of acute gouty arthritis(AGA).Methods According to the inclusion criteria,5 patients with AGA were enrolled in this study and treated with Qingre Tongluo Formula granule for 5 days.Five healthy subjects with non-gouty arthritis(HC)were selected without any treatment.LncRNAs were collected from peripheral blood mononuclear cells(PBMCS)of AGA patients,NAGA patients and HC patients,respectively.Arraystar LncRNAs Microarray technology was used to observe the phenotypic differences of LncRNAs among the three groups.Results Compared with HC group,a total of 2364 LncRNAs showed phenotypic differences in AGA group,of which 1174 were up-regulated and 1190 were down-regulated(Fold Change>2,P<0.05).Compared with HC group,a total of 802 LncRNAs were phenotypically different in NAGA group,including 147 up-regulated LncRNAs and 655 down-regulated LncRNAs(Fold Change>2,P<0.05).Compared with AGA group,171 LncRNAs showed phenotypic differences in NAGA group,including 30 up-regulated LncRNAs and 141 down-regulated LncRNAs(Fold Change>2,P<0.05).A total of 79 Incrnas were associated among AGA,NAGA and HC groups,among which 68 LncRNAs were up-regulated in AGA group and down-regulated in NAGA group,and 11 LncRNAs were down-regulated in AGA group and up-regulated in NAGA group.Conclusion The phenotypic dfferences of LncRNAs may be related to the acute onset of gout.
作者
张丽萍
何志艳
苏东文
鲍艳媛
谢招虎
向前
李兆福
ZHANG Liping;HE Zhiyan;SU Dongwen;BAO Yanyuan;XIE Zhaohu;XIANG Qian;LI Zhaofu(The First People's Hospital of Honghe Hani Yi Autonomous Prefecture,Gejiu 661000,China;Yunnan University of Chinese Medicine,Kunming 650500,China;The People's Hospital of Mile,Mile 652300,China;The First Affiliated Hospital of Yunnan University of Chinese Medicine,Kunming 650021,China)
出处
《云南中医药大学学报》
2023年第2期67-73,共7页
Journal of Yunnan University of Chinese Medicine
基金
国家自然科学基金项目(82260925,81760868)
云南省科学技术厅-云南中医药大学应用基础研究联合专项面上项目(202101AZ070001-018,202101AZ070001-241)
云南省高校中医风湿免疫病重点实验室(2018YGZ01)
云南省基础研究计划青年项目(202201AU070164)
云南省重点研发计划(202005AF150017)。
