摘要
为明确低分子量多环芳烃类化合物对水生生物的毒性作用,本研究探讨了菲(phenanthrene,Phe)对雌、雄性泥鳅(Misgurnus anguillicaudatus)肝脏结构及其相关酶活性的影响。将泥鳅分别静水暴露于1.29、1.67、2.16和2.79 mg·L^(-1)Phe 4个浓度的水溶液中,设置1个丙酮溶剂对照组、1个空白对照组,分别于处理7、14、21和28 d取样,采用赖氏比色法测定泥鳅肝脏谷草转氨酶(aspartate aminotransferase,AST)与谷丙转氨酶(alanine aminotransferase,ALT)活性的变化;制作胁迫14和28 d泥鳅肝脏组织切片,hematoxylin⁃eosin(HE)染色并进行观察。结果表明:与对照组相比,随着Phe胁迫浓度升高和时间延长,AST和ALT活性呈现显著的先升高后降低趋势,表现出一定的剂量效应和时间累计效应,且雌性泥鳅肝脏的AST和ALT活性均显著高于雄性泥鳅;肝脏组织学观察显示,1.29 mg·L^(-1)Phe胁迫14 d时,泥鳅肝细胞轻微肿大,少数肝细胞出现核溶解、变形、空泡化现象,2.79 mg·L^(-1)Phe胁迫28 d时,肝组织严重损伤,且雄性泥鳅肝脏结构受损更为严重;Phe在一定程度上对泥鳅肝脏AST和ALT具有先诱导后抑制的作用,且对雌性泥鳅影响更为显著;高浓度Phe持续胁迫可对肝组织产生明显损伤,且对雄性泥鳅肝损伤更为显著。
To clarify the toxic effects of low⁃molecular⁃weight polycyclic aromatic hydrocarbons on aquatic organ⁃isms,we examined the effects of phenanthrene(Phe)on the liver structure and related enzyme activities of female and male loach(Misgurnus anguillicaudatus).Loaches were exposed to different concentrations of Phe aqueous so⁃lution(1.29,1.67,2.16,and 2.79 mg·L^(-1),respectively)in hydrostatic water.Loaches that were kept in an ace⁃tone solvent were used as control,while those in deionized water were used as the blank control group.Loach sam⁃ples were harvested on day 7,14,21,and 28 after the treatments respectively.Rie’s colorimetric method was em⁃ployed to determine the changes of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)activi⁃ties in the liver of loach.The liver tissue slices of loaches under Phe stress for 14 and 28 days were prepared,stained with hematoxylin⁃eosin(HE)and observed.The results showed that compared with the control groups,the activities of AST and ALT exhibited a trend of significant increase and then decrease with the increases of Phe stress concentrations and treatment duration,showing a certain dose effect and time accumulation effect.In addition,the activities of AST and ALT of female loaches were significantly higher than those of males.Liver histological results showed that when loaches were treated by 1.29 mg·L^(-1)Phe for 14 days,their hepatocytes were slightly swelled,and a few hepatocytes appeared nuclear lysis,deformation and vacuolization.When loaches were treated by 2.79 mg·L^(-1)Phe for 28 days,their liver tissue was severely damaged,and the liver structure of the male was more se⁃verely damaged.Our results indicated that Phe can induce firstly and then inhibit AST and ALT in the liver of loach,and such effect is stronger in female loaches.Continuous stress of high⁃concentration Phe can cause signifi⁃cant damage to liver tissue,and the magnitude is more significant in male than female loaches.
作者
杨超超
谭婷
杨娜
王佳
吴航利
雷忻
YANG Chaochao;TAN Ting;YANG Na;WANG Jia;WU Hangli;LEI Xin(College of Life Sciences,Yan’an University,Yan’an Key Laboratory of Ecological Restoration,Yan’an 716000,Shaanxi,China)
出处
《生态学杂志》
CAS
CSCD
北大核心
2023年第6期1443-1448,共6页
Chinese Journal of Ecology
基金
陕西省自然科学基础研究计划面上项目(2021JM⁃417)
延安市科技惠民计划项目(2017HM⁃05)资助。
关键词
菲
泥鳅
肝脏
转氨酶
组织结构
phenanthrene
Misgunuis anguillicaudatus
liver
transaminase
histological structure
