摘要
目的:探讨依达拉奉右莰醇(C-EDA)对氧化应激以及自噬流的调控在周围神经损伤(PNI)中的作用。方法:PNI模型的建立和分组:将大鼠按照随机数字表法分为Sham组、PNI组、C-EDA组(C-EDA+PNI)、3MA(3-甲基腺嘌呤)组(PNI+C-EDA+3MA),每组10只。术后第4周对所有大鼠进行步态印记检测,神经电生理检测,腓肠肌湿重测量,肌肉组织HE染色,神经组织NF-200+MBP免疫荧光染色。用Western blot法检测体内的氧化应激相关蛋白Nrf2、HO-1,自噬相关蛋白LC3II/LC3I、p62,凋亡相关蛋白Bcl-2、Bax的表达情况。结果:与PNI组相比,C-EDA+PNI改善后足畸形,改善神经传导功能以及减轻腓肠肌萎缩。与PNI组相比,CEDA治疗上调体内的氧化应激相关蛋白Nrf2、HO-1、抗凋亡蛋白Bcl-2的表达,下调自噬底物蛋白p62、促凋亡蛋白Bax的表达(P<0.05)。3MA逆转了C-EDA改善损伤后的神经功能的效应。结论:C-EDA能够通畅自噬流,从而发挥改善PNI后神经结构修复与功能恢复的作用,是治疗PNI的一种潜在有效的方法。
Objective:To investigate the role of Edaravone Dexborneol(C-EDA)in regulating oxidative stress and autophagy flow in peripheral nerve injury(PNI).Methods:PNI model was established by moderate crush injury to the sciatic nerve with a vascular clamp.The rats were randomly divided into Sham group,PNI group,C-EDA group(C-EDA+PNI),and 3MA group[PNI+C-EDA+3MA(3-methyladenine)].At the 4th week after operation,gait imprinting,neuroelectrophysiological detection,wet weight measurement of gastnemius muscle,HE staining of muscle tissue and NF-200+MBP immunofluorescence staining of nerve tissue were performed in all rats.The levels of oxidative stress-related proteins Nrf2 and HO-1,autophagy related proteins LC3 II/LC3 I and p62(autophagy substrate protein,reflecting whether autophagy flow was unobstructed),apoptosisrelated proteins Bcl-2 and Bax were detected by Western blot.Results:Compared with the PNI group,C-EDA+PNI improved the posterior foot deformity,improved nerve conduction function and reduced gastrecnemius atrophy.3MA reversed the effect of C-EDA on improving neurological function after injury.Compared with PNI group,C-EDA up-regulated the expressions of oxidative stress-related proteins Nrf2,HO-1 and anti-apoptotic protein Bcl-2,and down-regulated the expressions of autophagy substrate protein p62 and pro-apoptotic protein Bax(P<0.05).Conclusion:Our study confirms that C-EDA can unobstruct autophagy flow,playing a role in improving neural structure repair and functional recovery after PNI.Therefore,it is a potentially effective method for the treatment of PNI.
作者
谌靖豪
楼承浩
魏圣哲
卢颖枫
余方正
王健
CHEN Jinghao;LOU Chenghao;WEI Shengzhe;LU Yingfeng;YU Fangzheng;WANG Jian(Department of Wound Repair,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,China;Department of Hand Surgery and Peripheral Neurosurgery,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,China;School of Pharmaceutical Science,Wenzhou Medical University,Wenzhou 325035,China)
出处
《温州医科大学学报》
CAS
2023年第7期517-525,共9页
Journal of Wenzhou Medical University
基金
国家自然科学基金项目(8217051594)。
关键词
周围神经损伤
依达拉奉右莰醇
氧化应激
自噬流
神经功能
peripheral nerve injury
Edaravon Dexborneol
oxidative stress
autophagy flow
neural function
